||4C & -20C
||10 pg / mL
||The Microwell Estradiol EIA is an enzyme immunoassay system for quantitative determination of Estradiol levels in Primate serum/plasma samples. The test is intended for professional use as an aid in the monitoring of conditions related to serum Estradiol. The test kit is designed to be used by a trained, skilled professional only.
1. Microtiter wells 96, coated with second antibody.
2. Enzyme Conjugate solution, 12 mL.(Blue Solution)
3. E2 Standard Set: 0, 10,30, 100, 300 and 1000, 5000 pg/mL.
4. Quality control set QC1, (80-100 pg/mL) and QC2, (600-800 pg/mL)
5. TMB Color Reagent, 12 mL
6. Stopping Solution (2N HCL), 6 mL
7. 20 X Wash Buffer, 20 mL (store at room temperature only).
Eatradiol is a steroid hormone with a molecular weight of 272, secreted from the testis in the male and by the ovary in non-pregnant women. In circulation Estradiol primarily isbound to the sex hormone binding globulin (SHBG) and is present 1-2% as free hormone. Eatradiol is metabolized in the liver to the more water soluble sulfate and glucuronide derivatives prior to excretion by the kidneys.
In women, the measurement ofEstradiol is useful in evaluating the status of ovarian functions hypo-estrogen in case of delayed puberty, primary and secondary amenorrhea and menopause. Estradiol levels in normal adult females change throughout the normal menstrual cycle.In theearly period of follicular development, the levels of Estradiol remain low. Approximately one week before the LH peak there is an initial decrease and then a more rapid rise of Estradiol, reaching a peak the day before the LH peak. Serum Estradiol canalso be used as a measure of the maturity of the ovarian follicle.
In men, elevated levels of Estradiol are often associated with gynecomestia, tenor of testis or liver, or cirrhosis of the liver. Both male and female infants have high concentrations of Estradiol in cord blood. Concentrations in both sexes during the first week of life are lower in cord serum, but higher than in later childhood, corresponding to the increased amounts of LH and FSH in infants at this time. During the first three days of life the percentage of free Estradiol is very high, more than twice that of sexually mature women. In normal prepubertal children, Estradiol is less than 10pg/ml. During puberty Estradiol increase progressively in girls, in parallelwith increases in gonadotropins. The importance of sequential Estradiol measurements for monitoring ovulation induction therapy, particularly in ''in vitro'' fertilization programs has recently been reported.
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