Estrone sulfate ELISA - Cosmo Bio Co.,Ltd.

Antibodies

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Estrone sulfate ELISA

Catalog No.: ENC-ERKP6006
Size: 1KIT
Price: ¥230000
$3067
antigen/source: Estrone sulfate
Description: standard: native
catalog info: Catalog 2012-p111
Storage: 4C
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Immunogen: Human
Reacts with: Human
Primates
Monkey
Sensitive: 0.2 ng / mL
Purpose: The Microwell Estrone Sulfate (ES) Elisa is an enzyme immunoassay system for quantitative determination of Estrone Sulfates levels in serum/plasma/urine samples of Primate and related species. The test is intended for professional use as research tool in monitoring of conditions related to serum/plasma/urine levels of Estrone Sulfates.
component: Antibody: X
Plate: X
Coating: X
Control: -
Standard: X
Labeling: X
Substrate: X
Others: X
1. Antibody-coated micro titer wells, 96-well plate
2. Reference Standard, 0.6ml (0, 0.05, 0.10, 1.0, 5.0, 25 ng/mL)
3. Enzyme Conjugate Reagent, 12 mL
4. TMB Color Reagent, 12 mL
5. 20X Wash buffer, 20 mL
6. Stop solution (2N HCl), 6mL
7. Instructions

Other:

 Supplementary 
Sandwich
 Applicable sample 
Serum, Plasma, Urine
[Other]
The ES quantitative test is based on a solid-phase enzyme immunoassay based on competitive binding method. A sample (serum/ plasma/urine) containing an unknown amount of ES to be assayed (unlabeled antigen) is added to a standard amount of a conjugated ES (labeled antigen). The labeled and unlabeled antigens are then allowed to compete for high affinity binding sites of ES antibody on coated on to the plate fortwo hours. After washing away the free antigen, the amount of labeled antigen in the sample is reversibly proportional to the concentration of the unlabeled antigen. The actual concentrations in unknown samples are obtained by means of a standard curve based on known concentrations of unlabeled antigen analyzed in parallel with the unknowns. After washing, substrate solution is added and the enzyme is allowed to react for a fixed time before the reaction is terminated. Absorbencies are measuredat 450 nm using ELISA plate reader. A standard curve is produced using values from 6 standards from which absorbency values for blank tubes have been subtracted. Results for unknown may be read directly from this standard curve using either manual calculation or by a suitable computer program. This kit is suitable for the direct measurement of ES in serum samples


Reference:

•  SE Shideler, NM Czekala, LH Kasman, 1983 - Soc Study Reprod
•  TE Ziegler, SA Sholl, G Scheffler, 1989 - Wiley Online Library
•  M Heistermann, S Tari, 1993 - Soc Reprod Fertility
•  Chattoraj SC 1976, NW Tietz eds.,WB Saunders, Chap 13, 699-823
•  Makoto Hirako et al., 2003 Jap. Int.Res. Cen Ag. Sci 37, (3) p1-9
•  Horne C. et al., 1983 Biol Reprod 29 p56-62
•  JK Hodges, SAK Eastman - American Journal of Primatology, 1984 - Wiley Online Library
 
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