Progesterone (serum) ELISA
||4C & -20C
||0.1 ng / mL
||The Microwell Progesterone ELISA is an enzyme immunoassay system for quantitative determination of progesterone levels in serum/plasma. The test is intended for professional use as a research tool in the monitoring of conditions related to serum progesterone.
1. Wells coated with progesterone antibody (96 wells)
2. Enzyme Conjugate, 12 mL
3. Progesterone Standard Set: 0, 1, 2.5, 5, 10, 30 ng /ml 0.5 ml / Vial
4. TMB Color Reagent, 12 mL
5. Stop Solution (2N HCL), 6 mL.
6. 20 X Wash Buffer, 20mL.
Progesterone is a steroid hormone (C21 steroid, pregn-4-ene-3, 20 dione) and is synthesized from both tissue and circulating cholesterol. The principal production sites are the adrenals and ovaries and placenta during pregnancy. The majority of this steroid is metabolized in the liver to pregnanediol and conjugated as a glucuronide prior to excretion by kidneys. The primary role is played in reproductive organs. In males the progesterone plays an intermediary role in the synthesis of corticosteroids and androgens. In females, progesterone remains relatively constant through follicular phase of ovulation. Then the levels increase following ovulation and remain elevated for several hours and decreasing to the base line 24 hours before the onset of cycle. In pregnancy, placental progesterone raise 10-30 times those of the luteal peak levels.
In females, the measurement of progesterone is a useful marker in evaluating the status of ovarian functions and also pregnancy.
• Kasson BG. et al.,. 1993, J Immunoassay 14 33-49
• Roby KF. 2001, Endocrinology 142 2328
• Vaitukaitis J. Robbins JB. Nieshlag E. Ross GT J. Clin. Endocrinol. Metab. 33 988-991. (1971)
• Nakane PK. and Kawaoi A. J. Histochem. Cytochem. 22:1084-1091(1974).
• Maiolini R. and Masseyeff R. J. Immunol. Methods. 8:223-234 (1975).
• Hsu, YH. Anal. Biochem. 142:221-225, (1984).
• Chattoraj SC 1976 Endocrine function in Fundamentals of Clinical Chemistry, NW Tietz eds WB Saunders Chap 13, 699-823