Thyroid Stimulating Hormone (TSH) ELISA - Cosmo Bio Co.,Ltd.

Antibodies

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Thyroid Stimulating Hormone (TSH) ELISA

Catalog No.: ENC-ERKP6015
Size: 1KIT
Price: ¥230000
$3067
antigen/source: Thyroid Stimulating Hormone
Description: standard: native
catalog info: 2008 Japanes Assay kit Catalog-p833
Storage: 4C & -20C
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Immunogen: Human
Reacts with: Human
Primates
Monkey
Sensitive: 1 ng / mL
Purpose: The PRIMATE TSH ELISA test is an immunoassay designed for the quantitative determination of thyroid stimulating hormone (TSH) in serum/plasma samples of PRIMATE and related species.
component: Antibody: X
Plate: X
Coating: X
Control: -
Standard: X
Labeling: X
Substrate: X
Others: X
1. Antibody-coated microtiter wells
2. Reference standard, Ready to use (0, 1, 2.5, 5, 10, 25, ng/mL), 0.6mL/vial
3. Enzyme Conjugate Reagent, 12 mL
4. TMB color reagent (ready to use) , 12 mL
5. 20X Wash buffer, 20 mL
6. Stop solution (2N HCl), 6mL
7. Instructions

Other:

 Supplementary 
Sandwich
 Applicable sample 
Serum, Plasm
[Other]
TEST PRINCIPLE
The PRIMATE TSH ELISA test is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay system utilizes affinity purified antibody directed against intact PRIMATE TSH molecule for solid phase (microtiter wells) immobilization and a mouse anti bovine TSH beta antibody is in the antibody-enzyme (horseradish peroxidase) conjugate. The test sample is allowed to react simultaneously withthe two antibodies, resulting in the TSH molecules being sandwiched between the solid phase and enzyme-linked antibodies. After a 1 hours of incubation period at 37 C, the wells are washed with wash solution to remove unbound-labeled antibodies. A solution of TMB is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of stop solution and the color is changed to yellow and measured spectrophotometrically at 450 nm. The concentration of TSH is directly proportional to the color intensity of the test sample.


Reference:

•  Ferguson DC 1994 Vet Clic North Am Small Anim Prac 24(3):p515-539
•  Reimers TJet al.,1990 Am J Vet Res 51(3)p454-457
•  Panceiera DL, 1999 J Small Anim Pract 40 (4)p152-157
•  Dixon RM and Mooney CT, 1999 J Small Anim. Pract 40(2) P72-78
•  Bruner, JM et al., 1998 J Am Vet Med. Assoc. 15 212(10)., p1572-1575
•  Scott-Moncrieff JCet al.,1998 J Am Vet, Med. Assoc. 1;212(3):p387-391
•  Williams Daet al.,1996 J Am Vet Med Assoc. 15,209(10):p1730-1732
•  Walker W.H.C. Introduction: An Approach to Immunoassay. Clin. Chem. 1977; 23: 384
•  Wisdom G.B. Enzyme Immunoassay. Clin. Chem. 1976; 22: 1243
 
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