||2008 Japanes Assay kit Catalog-p695
||4C & -20C
||The Aldosterone ELISA test is an immunoassay designed for the quantitative determination of Aldosterone in serum/plasma. The test is intended for professional use as an aid in the diagnosis and monitoring of physiological/pathological conditions related to serum/plasma Aldosterone in Canine and related species.
1. Microtiter wells coated with Aldosterone specific antibody.
2. Enzyme labeled (HRP) Aldosterone solution, 12mL
3. Aldosterone Standards 1 Set ready to use, 0.5ml/vial(Contains 0, 10, 50,200,1000,5000,10,000 pg/ml)
4. TMB Color Reagent (One-step ready to use), 12 mL
5. Stop Solution (2N HCl), 6 mL
6. 20x Wash Buffer, 20 mL.
7. Sample diluent, 20mL
The Aldosterone Quantitative Test is based on a widely used immunoassay technique. A sample (serum/ plasma) containing an unknown amount of Aldosterone to be assayed (unlabeled antigen) is added to a standard amount of a labeled derivative of the same substance (labeled antigen). The labeled and unlabeled antigens are then allowed to compete for high affinity bindin sites on a limited number of antibodies coated on tothe plate. After washing away the free antigen, the amount of labeled antigen in the sample is reversibly proportional to the concentration of the unlabeled antigen. The actual concentrations in unknown samples are obtained by means of a standard curve based on known concentrations of unlabeled antigen analyzed in parallel with the unknowns. In this kit an enzyme label is used. The biospecfic reaction takes place during 2hour incubation. After washing away, substrate solution is added and the enzyme allowed to react for a fixed time before the reaction is terminated. Absorbencies are measured at 450 nm using ELISA plate reader. A standard curve is produced using values from 6 standards from which absorbency values for blank tubes have beensubtracted. Results for unknown may be read directly from this standard curve using either manual calculation or by a suitable computer program.
This kit is suitable for the direct measurement of Aldosterone in serum/plasma samples of K9 and related species. It may also be used following an extraction procedure for assaying urinary Aldosterone (call for details of the procedure). Note: The Aldosterone levels should be established in your laboratory using your own set of samples and standards andgood laboratory practice should be employed where applicable.
NOTE: THIS IS SPECIFICALLY DESIGNED TO MEASURE ALDOSTERONE IN CANINE AND RELATED SPECIES. ALL HUMAN ALDOSTERONE KITS AVAILABLE IN THE MARKET DO ADDRESS THE INTERFERENCE OF
• BONDY PK. The adrenal cortex, in Randy PT, Rosenberg LE., Metabolic control and disease (8 ed) 1980, WB Sanders, Philadelphia, p1427-1499.
• Lambert A Clinical Endocrinology 1974, Springer-Verlag New York, p299-305
• Veosei P, Glucocorticoids, Aldosterone, corticosterone, Compound S in Jaffe BM, Behrman HR 1974 in Methods in Radioimmuno assay p393-411.
• Spark R 1971, Simplified assessment of pituitary-adrenal reserve Annals of internal Med. 75 p75-717