||Follicle Stimulating Hormone (FSH)
||4C & -20C
||1 ng / mL
||The FSH ELISA Test is an immunoassay designed for the quantitative determination of follicle-stimulating hormone concentrations in serum. The test is designed for professional use only and should be employed by a trained/skilled professional. The assay is designed to measure circulating levels of FSH in Rodent and related species.
1. Antibody-coated microtiter wells, 96-well plate
2. Enzyme Conjugate Reagent, 12 mL
3. TMB Color Reagent, 12 mL
4. 20X Wash buffer, 20 mL
5. Stop solution (2N HCl), 6mL
6. Reference Standard, 0.8ml (0, 1.0, 2.5, 5.0, 10, 25 ng/mL) QC Level 1 (2-5ng/ml) and QC Level 2 (10-15 ng/mL)
Follicle-Stimulating Hormone (FSH) and Luteinizing Hormone (LH) are intimately involved in the control of the growth and reproductive activities of the gonadal tissues, which synthesizeand secrete male and female sex hormones. The levels of circulating FSH and LH are controlled by these sex hormones through a negative feedback relationship. FSH is a glycoprotein secreted by the basophilic cells of the anterior pituitary. Gonadotropin-release hormone (GnRH), produced in the hypothalamus, controls the release of FSH from the anterior pituitary. Like other glycoproteins, such as LH, TSH, and hCG, FSH consists of subunits designated as alpha and beta. Hormones of this type have alpha subunits that are very similar structurally, therefore the biological and immunological properties of each are dependent on the unique beta subunit. In the female, FSH stimulates the growth and maturation of ovarian follicles by acting directly on the receptors located on the grannulosa cells; follicular steroidogenesis is promoted and LH production is stimulated. The LH produced then binds to the theca cells and stimulates steroidogenesis. Increased intraovarian Estradiol production occursas follicular maturation advances, thereupon stimulating increased FSH receptor activity and FSH follicular binding. FSH, LH, and Estradiol are therefore intimately related in supporting ovarian recruitment and maturation of the ovum in female.
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