||4C & -20C
||0.1 ng / mL
||The Microwell Progesterone ELISA is an enzyme immunoassay system for quantitative determination of progesterone levels in serum/plasma. The test is intended for professional use as a research tool in the monitoring of conditions related to serum progesterone in rodent and related species.
1. Wells coated with progesterone antibody (96 wells)
2. Enzyme Conjugate, 12 mL
3. Sample/standard diluent 25 mL
4. TMB Color Reagent, 12 mL
5. Stop Solution (2N HCL), 6 mL.
6. 20 X Wash Buffer, 20 mL.
7. Progesterone Standard Set: 0, 1.0, 2.5, 5.0, 10, 30 ng / mL 0.6 ml / Vial
Progesterone is a steroid hormone (C21 steroid, pregn-4-ene-3, 20 dione) and is synthesized from both tissue and circulating cholesterol. The principal production sites are the adrenals and ovaries and placenta during pregnancy. The majority of this steroid is metabolized in the liver to pregnanediol and conjugated as a glucuronide prior to excretion by kidneys. The primary role is played in reproductive organs. In males the progesterone plays an intermediary role in the synthesis of corticosteroids and androgens. In females, progesterone remains relatively constant through follicular phase of ovulation. Then the levels increase following ovulation and remains elevatedfor several hours and decreasing to the base line 24 hours before the onset of cycle. In pregnancy, placental progesterone raise 10-30 times those of the luteal peak levels. In female rodents, the measurement of progesterone is an useful marker in evaluating the status of ovarian functions and also pregnancy.
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