T3 ELISA - Cosmo Bio Co.,Ltd.

Antibodies

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T3 ELISA

Catalog No.: ENC-ERKR7013
Size: 1KIT
Price: ¥125000
$1667
antigen/source: T3
Description: standard: native
catalog info: Catalog 2012-p113
Storage: 4C & -20C
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Immunogen: Rodent
Reacts with: Mouse
Rat
Sensitive: 0.2 ng / mL
Measurement Range: 0.5-10ng/mL
Purpose: The T3 ELISA test is an immunoassay designed for the quantitative determination of Triiodothyronine (T3) in serum/plasma samples of Rodent and related species.
component: Antibody: X
Plate: X
Coating: X
Control: -
Standard: X
Labeling: X
Substrate: X
Others: X
1. Antibody-coated microtiter wells, 96-well plate
2. T3 HRP Conjugate, 12mL
3. Reference Standard Ready to use liquids 0.5 mL/Vial, (0,0.5, 1, 2.5, 5, 10, 20 ng / mL)
4. TMB Color Reagent (Ready to use) 12 mL
5. Stop solution (2N HCl) 6 mL
6. 20X Wash Buffer , 20 mL
7. Sample diluent, 10 mL
8. Instructions

Other:

 Supplementary 
Sandwich
 Applicable sample 
Serum, Plasma
[Other]
TEST PRINCIPLE
In the T3 ELISA Test, anti T3 antibody is coated on microtiter wells. A measured amount of Swine serum/plasma and a constant amount of T3 conjugated with horseradish peroxidase are added to the microtiter wells. During one hour incubation at 37C, the T3 and conjugated T3 compete for the limited binding sites on the anti-T3 antibody on the wells. After the incubation period, the wells are washed 5 timeswith wash buffer to remove any unbound T3 conjugate. A solution of TMB subtract is then added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of stop solution and the absorbencyis measured spectrophotometrically at 450nm. The intensity of the color formed is proportional to the amount of enzyme present and is inversely related to the amount of unlabeled T3 in the sample. By reference to a series of T3 standards assayed in the same way, the concentration of T3 in the unknown sample is quantified.


Reference:

•  Dauncey MJ and Morovat A, 1993, J Endocrinol 139(1), p131-141
•  Djurdjevic Dj et al., 1992 Glas Srp.Akad Nauka (42)p123-129
•  Lefaucheur L and Etienne M., 1991 Domest animal Endocrinol Oct 8(4) p601-609
•  Martin RJ et al., 1984 Growth 48(2)p158-165
•  Slebodzinski AB and Cogiel F 1983 Endocrinol Exp 17(3-4)p263-270
•  Slebodzinski AB et al., 1981Bio Neonate 39(3-4)p191-199
•  Walker W.H.C. Introduction: An Approach to Immunoassay. Clin. Chem. 1977; 23: 384
•  Wisdom G.B. Enzyme-Immunoassay. Clin. Chem. 1976; 22: 1243
•  Hoffenberg R. Medicine 1978; 8: 392
10. Lieblich J., Utiger R.D. J. Clin. Invest. 1972; 51: 1939
 
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