TSH ELISA - Cosmo Bio Co.,Ltd.

Antibodies

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TSH ELISA

Catalog No.: ENC-ERKR7015
Size: 1KIT
Price: ¥125000
$1667
antigen/source: TSH
Description: standard: native
catalog info: 2008 Japanes Assay kit Catalog-p1012
Storage: 4C & -20C
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Immunogen: Rodent
Reacts with: Mouse
Rat
Sensitive: 0.2 ng / mL
Purpose: The Rodent TSH ELISA test is an immunoassay designed for the quantitative determination of thyroid stimulating hormone (TSH) in serum/plasma samples of rat/mouse and related species.
component: Antibody: X
Plate: X
Coating: X
Control: -
Standard: X
Labeling: X
Substrate: X
Others: X
1. Antibody-coated microtiter wells
2. Reference standard Lyophilized set (0, 1, 2.5, 5, 10, 25 ng / mL).
3. Enzyme Conjugate, 1 Vial Orange Cap, reconstitute in 12 mL
4. TMB color reagent (ready to use), 12 mL
5. 20X Wash buffer, 20 mL
6. Stop solution (2N HCl), 6 mL
7. Standard/Sample Diluent, 20 ml

Other:

 Supplementary 
Sandwich
 Applicable sample 
Serum, Plasma
[Other]
TEST PRINCIPLE:
The Rodent TSH ELISA test is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay system utilizes affinity purified antibody directed against intact rodent TSH molecule for solid phase (microtiter wells) immobilization and a mouse anti-TSH antibody is in the antibody-enzyme (horseradish peroxidase) conjugate. The test sample is allowed to react simultaneously with the two antibodies, resulting in the TSH molecules being sandwiched between the solid phase and enzyme-linked antibodies. After 3 hours of incubation period at 37 C, the wells are washed with wash solution to remove unbound-labeled antibodies. A solution of TMB is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of stop solution and the color is changed to yellow and measured spectrophotometrically at 450 nm. The concentration of TSH is directly proportional to the color intensity of the test sample.


Reference:

•  Iyengar MR, Coleman DW, Butler TM. Phosphocreatinine, a high-energy phosphate in muscle, spontaneously forms phosphocreatine and creatinine under physiological conditions. J Biol Chem. 1985 Jun 25;260(12):7562-7.
•  Furter R, Kaldis P, Furter-Graves EM, Schnyder T, Eppenberger HM, Wallimann T. Expression of active octameric chicken cardiac mitochondrial creatine kinase in Escherichia coli. Biochem J. 1992 Dec 15;288 ( Pt 3):771-5.
•  Wang ZM, Gallagher D, Nelson ME, Matthews DE, HeymsfieldSB. Total-body skeletal muscle mass: evaluation of 24-h urinary creatinine excretion by computerized axial tomography. Am J Clin Nutr. 1996 Jun;63(6):863-9.
•  Wyss M, Kaddurah-Daouk R. Creatine and creatinine metabolism. Physiol Rev. 2000 Jul;80(3):1107-213. Review.
•  Miller RC, Brindle E, Holman DJ, Shofer J, Klein NA, Soules MR, O'Connor KA. Comparison of specific gravity and creatinine for normalizing urinary reproductive hormone concentrations. Clin Chem. 2004 May;50(5):924-32. Epub 2004Mar 11.
 
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