||4C & -20C
||1 ng / mL
||The Microwell Progesterone ELISA is an enzyme immunoassay system for quantitative determination of progesterone levels in serum/plasma. The test is intended for professional use as an aid in the diagnosis and monitoring of conditions related to serum progesterone in swine and related species.
1. Wells coated with progesterone antibody , 96 wells
2. Enzyme Conjugate, 12mL
3. Progesterone Standard Set: 0, 1.0, 2.5, 5.0, 10, 30 ng / mL QC1 (<2.5ng/mL) QC2 (10ng/mL)
4. Sample/standard diluent 25mL
5. TMB Color Reagent, 12 mL
6. Stop Solution (2N HCL), 6mL.
7. 20 X Wash Buffer, 20mL.
Progesterone is a steroid hormone (C21steroid, pregn-4-ene-3, 20 dione) and is synthesized from both tissue and circulating cholesterol. The principal production sites arethe adrenals and ovaries and placenta during pregnancy. The majority of this steroid is metabolized in the liver to pregnanediol and conjugated as a glucuronide prior to excretion by kidneys. The primary role is played in reproductive organs. In males the progesterone plays an intermediary role in the synthesis of corticosteroids and androgens. In females, progesterone remains relatively constant through follicular phase of ovulation. Then the levels increase following ovulation and remains elevated for 4-6 days decreasing to the base line 24 hours before the onset of cycle. In pregnancy, placental progesterone raise 10-30 times those of the luteal peak levels. In females, the measurement of progesterone is useful in evaluating the status of ovarian functions. Monitoring of progesterone therapy and early stage pregnancy evaluations comprise the remainder of progesterone assays. During early ovarian maturation progesterone levels increase progressively in girls, in parallel with increases in gonadotropins. The importance of sequential progesterone measurements for monitoring ovulation induction, particularly in ''in vitro'' fertilization programs has recently been reported. The monitoring of LH and progesterone will help the breeder
• Zanella E, Lunstra D, Wise T, Kinder J, Ford J. Biol Reprod 1999 Jan;60(1):115-118
• Colenbrander B, Van de Wiel DF, van Rossum-Kok CM, Wensing CJ. Biol Reprod 1982 Feb;26(1):105-109
• Wise T, Lunstra DD, Ford JJ. Biol Reprod 1996 Jan;54(1):146-153
• Kermabon AY, Prunier A, Djiane J, Salesse R. Biol Reprod 1995 Nov;53(5):1095-1102.
• Shaw HJ, Foxcroft GR. J Reprod Fertil 1985 Sep;75(1):17-28
• Guthrie HD, Bolt DJ. J Anim Sci 1983 Oct;57(4):993-1000
• Diekman MA, Trout WE, Anderson LL. J Anim Sci 1983 Jan;56(1):139-145
• Marshall J.C. Clinic in Endocrinol. Metab. 1975; 4:454
• Cohen K.L. Mtabolism 1977; 26:1165
• Uotila M., Ruoslahti E. and Engvall E. J. Immunol. Methods 1981; 42:11
• Autrere MB and Benson H 1976 Progesterone: an overview and recent advances Jour Par Sci 65, (6)783-800
• Chattoraj SC 1976 Endocrine function in Fundamentals of Clinical Chemistry, NW Tietz eds,WB Saunders Chap 13, 699-823 Revised: 0810