||1.563 - 100 ng/mL
||This EIA kit is used for quantitative determination of Ucn 1 in mouse/rat plasma and serum samples. The kit is characterized by its sensitive quantification and high specificity. In addition, it has no influence by other components in samples. Mouse/rat Ucn1 standard is highly purified synthetic product.
1. Antibody coated plate 1 plate (96 wells)
2. Standard 1 vial (100 ng )
3. Labeled antigen 1 vial
4. SA-HRP solution 1 bottle (12 mL )
5. Enzyme substrate solution 1 bottle (12 mL )
6. Stopping solution 1 bottle (12 mL )
7. Buffer solution 1 bottle (15 mL)
8. Washing solution (concentrated) 1 bottle (50 mL )
9. Adhesive foil 3 pieces
Urocortin (Urocortin1: Ucn1) is first identified in rat, and later in human and mouse. It is the second mammalian member of the CRF family. Rat and mouse Ucn1 have the same amino acid sequence and display 95% structure homology to human Ucn1, 45% to CRF and 63% to urotensin. In the rat, Ucn1 immunoreactivity (IR) was shown to distribute widely in central nervous system, endocrine organs, and digestive system and its concentration was highest in pituitary (11 pmol/g, w.w.). Koziez, Yanaihara et al. used a polyclonal antibody against rat Ucn1 to define the distribution of Ucn1-IR in rat central nervous system and found a large number of neurons with Ucn1-IR in rat brain.
Synthetic human Ucn1 binds with high affinity to CRF receptor type 1(CRFR1), 2 alpha(CRFR2..) and 2 beta (CRFR2..). CRFR1 and CRFR2 have been shown to link to the development of stress-related disorders, such as mood and eating disorders.CRFR1 is expressed predominantly in the brain and pituitary, whereas CRFR2 expression is limited to particular brain areas and to some peripheral organs. Data were also presented supporting the hypothesis that this peptide is the endogenous ligand for the CRFR2. Synthetic human Ucn1 stimulates cAMP accumulation in cells stably transfected with those receptors and acts in vitro to release ACTH from dispersed rat anterior pituitary cells. In addition, the CRF-binding protein binds human Ucn1 withhigh affinity and can prevent Ucn1-stimulated ACTH secretion in vitro. Ucn1 was suggested to play important roles in various tissues in normal rats, but shown not to behave as a hypothalamic hypophysiotropic factor in mediating adrenocorticotropin secretin in adrenalectomized rats. Ucn1 has been implicated in various endocrine responses, such as blood pressure regulation, as well as in higher cognitive functions.
Synthetic human Ucn1 also stimulates plasma ACTH, cortisol and atrial natriureticpeptide (ANP) secretin and suppresses plasma ghrelin in healthy male volunteers In the human, plasma Ucn1 is elevated in heart failure, especially in its early stages. This fact may useful in the diagnosis of early heart failure.
We have already developed mouse urocortin 2 (Ucn2) EIA kit (YII-YK190-EX), rat urocortin 2 (Ucn2) EIA kit (YII-YK191-EX) and mouse/rat urocortin 3 (Ucn3) EIA kit (YII-YK200-EX). This time, as a part of tools for urocortin research, our laboratory developed mouse/rat Ucn1 EIA kit (YII-YK210-EX), which is highly specific for mouse/rat Ucn1 with almost no crossreaction with Ucn2 (mouse), Ucn2 (rat), Ucn3 (mouse, rat), ACTH (mouse, rat), ACTH (human) and CRF (mouse, rat, human). The kit can be used for measurement of Ucn1 inmouse/rat plasma or serum with high sensitivity. It will be a specifically useful tool for Ucn1 research.
• Reyes TM. (2001) Urocortin II: a member of the corticotropin-releasing factor (CRF) neuropeptide family that is selectively bound by type 2 CRF receptors. Proc Natl Acad Sci USA. 98, 2843-2848
• Chen A. (2003) Glucocorticoids regulate the expression of the mouse urocortin II gene: a putative connection between the corticotropin-releasing factor receptor pathways. Mol Endocrinol.,17, 1622-1639
• Chen A. (2004) Urocortin II gene is highly expressed in mouse skin and skeletal muscle tissues: localization, basal expression in corticotropin-releasing factor receptor (CRFR) 1-and CRFR2-null mice, and regulation by glucocorticoids. Endocrinology., 145, 2445-2457
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• Bale TL. (2004) The cardiovascular physiologic actions of urocortin II: acute effects in murine heart failure. Proc Natl acad Sci U S A., 101, 3697-3702
• Brar BK. (2004) Urocortin II and urocortin III are cardioprotective against ischemia reperfusion injury: an essential endogenous cardioprotective role for corticotropin releasing factor receptor type 2 in the murine heart. Endocrinology., 145,24-35
• Hinkle RT. (2003) Urocortin II treatment reduces skeletal muscle mass and function loss during atrophy and increases nonatrophying skeletal muscle mass and function. Endocrinology., 144, 4939-4946