rZDSN (Zuwai crab duplex-specific nuclease) - Cosmo Bio Co.,Ltd.

Antibodies

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rZDSN (Zuwai crab duplex-specific nuclease)

Catalog No.: CSR-BZN103
Size: 100UNIT
[100 ul]
Price: [DISCONTINUED] Sorry!! This product is not available anymore.
Description: Recombinant, Baculovirus
catalog info: Catalog 2012-p195
Storage: -20C
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Purpose: Duplex-specific nuclease purified from insect cells infected by recombinant baculovirus containing Chionoecetes opilio (Zuwai crab or snow crab) hepatopancreas cDNA.
component: [Volume]
BZN102 : 50ul , BZN103 : 100ul
[Source]
Purified from insect cells infected by recombinant baculovirus containing Chionoecetes opilio (Zuwai crab or snow crab) hepatopancreas cDNA for duplex-specific nuclease [5].

Other:

 Background 
The rZDSN degrades double-stranded DNA and DNA in DNA-RNA hybrid duplex with high selectivity, while displays little cleaving activities to single-stranded DNA. The rZDSN is a thermostable enzyme with an optimum temperature around 60C. The rZDSN is tolerant to a proteinase K digestion. The duplex-specific nuclease activity is applicable to various purposes, for example, methods for SNP analysis (1), cDNA normalization (2), subtraction (3), and quantitative telomeric overhang determination (4).


Reference:

1. Shagin DA, et al., Genome Res, 12(12), 1935-1942 (2002).
2. Zhulidov PA, et al., Nucleic Acids Res, 32(3), e37 (2004).
3. Peng RH, et al., Anal Biochem, 372(2), 148-155 (2008).
4. Zhao Y, et al., Nucleic Acids Res, 36(3), e14 (2008).
5. Hirakawa Y, Ohiso I, Cloning, expression, and characterization of thermostable duplex-specific nuclease from snow crab (Chionoecetes opilio). The 32nd Annual Meeting of the Molecular Biology of Japan (MBSJ2009).
 
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