Tma endoV M2-6 (mutant Thermotoga maritima endonuclease V) - Cosmo Bio Co.,Ltd.

Antibodies

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Tma endoV M2-6 (mutant Thermotoga maritima endonuclease V)

Catalog No.: CSR-EMV001
Size: 100UNIT
[100 ul]
Price: [DISCONTINUED] Sorry!! This product is not available anymore.
Description: Recombinant, E.coli
catalog info: Catalog 2012-p195
Storage: -20C
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Purpose: Mutant Endonuclease
component: [Buffer composition]25 mM HEPES (pH7.4), 0.5 mM EDTA , 50 uM DTT, 25 mM NaCl, 250 mM, imidazole, 50% (v/v) glycerol, 10x Reaction buffer 1 ml (100 mM HEPES (pH7.4)C50 mM MgCl2C10 mM DTT)
[Molecular Weight]28kDa (SDS-PAGE)

Other:

 Background 
Tma endoV M2-6 is an endonuclease having modified substrate specificity created by amino acid substitutions to Tma endoV (endonuclease V from thermophilic bacterium Thermotoga maritima). TmaendoV has a deoxyinosine 3'-endonuclease activity, i.e. it recognizes a hypoxanthine (the deoxyinosine base) and exhibits a nicking activity to hydrolyze the second phosphodiester bond to 3' the lesion base(1-4). The wild-type endoV also shows nonspecific DNA cleaving activities, independent of the specific recognition of the lesion structure. Tma endoV M2-6 exhibits a significantly reduced nonspecific activity toward an intact DNA and retains the specific endonuclease activity against a deoxyinosine-containing DNA.
[Producttype]
Endonucelase V
[Source]
Purified from Escherichia coli cells carrying a mutant endonuclease V gene (nfi gene), which was cloned from Thermotoga maritima and introduced site-directed mutagenesis
[NOTE] This product cannot be sold to USA because of the patent issue.



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Reference:

1.Huang J, et al., Biochemistry, 40(30), 8738-8748 (2001).
2) Huang J, et al., Biochemistry, 41(26), 8342-8350 (2002).
3) Hitchcock TM, et al., Nucleic Acids Res, 32(13), 4071-4080 (2004).
4) Feng H, et al., Biochemistry, 44(34):11486-11495 (2005).
5) Nakashima K, Takano F, Matsuo K, Ohiso I, Highly specific recognition and cleavage of deoxyinosine-containing DNA by mutant Thermotoga maritima endonuclease V. The 32nd Annual Meeting of the Molecular Biology of Japan (MBSJ2009).
 
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