Rat/Mouse Surfactant Protein D ELISA Kit - Cosmo Bio Co.,Ltd.

Antibodies

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Rat/Mouse Surfactant Protein D ELISA Kit

Catalog No.: CSR-80072
Size: 1KIT
[96 tests]
Price: see also
#YMS-80072EX
Size 96
antigen/source: Rat/Mouse SP-D
Storage: 4C
@
Reacts with: Rat
Mouse
Measurement Range: 0.47H30 ng / mL
Purpose: Measurement of Rat/Mouse SP-D
component: Antibody: X
Plate: X
Coating: X
Control:
Standard: X
Labeling:
Substrate: X
Others: X
1. Antibody Coated Plate (96 microwells plate, 8wells x 12 strips)...1 set
2. Rat SP-D Standerd 1 (0.47 ng/mL, 0.5mL)...1 vial
3. Rat SP-D Standerd 2 (1.88 ng/mL, 0.5mL)...1 vial
4. Rat SP-D Standerd 3 (7.5 ng/mL, 0.5mL)...1 vial
5. Rat SP-D Standerd 4 (30 ng/mL, 0.5mL)...1 vial
6. Sample Diluent (50mL)...1 vial
7. Concentrated Washing Solution (50mL)...1 vial
8. Enzyme Conjugate (0.15mL)...1 vial
9. Enzyme Conjugate Diluent (15mL)...1 vial
10. Color Developing Reagent A (11mL)...1 vial
11. Color Developing Reagent B (0.5mL)...1 vial
12. Stop Solution (11mL)...1 vial

Other:

 Supplementary 
Sandwich
 Applicable sample 
DNA
 Principle 
This kit is a solid phase enzyme-linked immunosorbent assay (ELISA) using two monoclonal antibodies against rat SP-D and rat SP-D as a standard material. Both antibodies also can react to mouse SP-D. It is possible to measure the concentration of rat and mouse SP-D in serum and/or bronchoalveolar lavage fluid (BALF) by this kit.
[Measurement range]
0.47 - 30 ng/mL.



 IMAGE1



Bleomycin (1 mg/kg) was administered intratracheally to male Wistar Rats (8 weeks-old). Serum and bronchoalveolar lavage fluid (BALF) samples were collected from day 0 to 28 after bleomycin treatment. Serum SP-D concentrations of bleomycin-treated ratswere elevated from day 5 and peaked on day 10 , then gradually decreased until day 28. Serum samples and BALF samples in controlrats were not elevated. In the case of serum aspartate aminotransferase and C-reactive protein, there was no significant difference between bleomycin-treated rats and control rats.

 IMAGE2



Lungs prepared from normal rats and bleomycin-treated rats were fixed in 20% formalin solution and embedded in paraffin. These samples were stained with hematoxylin and eosin for histological evaluation. Inflammatory cell infiltration and pulmonary edema were observed in cell wall after day 3. On day 10 when serum SP-D concentration of bleomycin-treated rats were peaked, the density of inflammatory cell infiltration was the highest of other points and alveolar collapse was recognized. After day 28, SP-D concentration of serum and BALF were decreased and inflammatory cell infiltration and alveolar collapse were improved. These results showed that concentration of serum SP-D in bleomycin-treated rats corresponds with inflammation in lung.

Reference:

•  Murata M. et al., Exp Lung Res 36 463-468 (2010)
•  Murata M. et al., J Toxical Sci. 35 Supplement P.S208
 
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