Streptolysin O - Cosmo Bio Co.,Ltd.

Antibodies

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Streptolysin O

Catalog No.: BAM-01-531-EX
Size: 20UG
[1 mg / ml]
Price: [DISCONTINUED] Sorry!! This product is not available anymore.
Description: Recombinant, Ecoli[Species]Hemolytic streptococcus
Application: Western Blot
Enzyme Linked Immunosorbent Assay
catalog info: Catalog 2012-p169
Storage: -20C
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Immunogen: Microbes
Purity: >98% by SDS-PAGE
Shape: Steri. and Liq.
Purpose: [Application]
> Antigen for the measurement of anti-streptolysin 0 antibody (AS0) (diagnostic reagent).
> Reagent for membrane pore formation to introduce small to macromolecules into living cells.
component: [Purity]
Over 98% is SLO by SDS-PAGE
[Form]
1 mg / ml in PBS (-), 1 mM DTT, 50% glycerol, sterilized by filtration

Other:

 Background 
Streptolysin 0 (SL0) is a membrane-damaging extracellular toxin produced by hemolytic streptococci. The membrane-damaging activity is measured by hemolysis of red-blood cells. SL0 is oxygen-sensitive and is easily inactivated in itspresence but can be reactivated by thiol compounds, thus is referred also as called thiol-activated cytolysin (2). SL0 is produced not only by Group A hemolytic streptococci but also by Group C and Group G strains. The amino acid sequences are highlyconservedamong them and their homology is over 98%.
The product was highly purified from E.coli over-expressing SL0 of Group C hemolytic streptococci. The specific activity is as high as >900,000 hemolytic units (HU) / mg and Forms a big hole on the cell membrane, which enables the introduction of protein inside the cells in vivo (1).



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Reference:

•  U Walev I.et al.,Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O,PNAS, 98:3185 (2001)
•  M. Palmer: The family of thiol-activated, cholesterol-binding cytolysins, Toxicon, 39, 1681-1689 (2001)
•  Maeda, Y.et al.,''GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus.'' Nat. Cell Biol. 10: 1135-45 (2008)
 
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