Pfu DNA polymerase (+dNTPs), Economy - Cosmo Bio Co.,Ltd.

Antibodies

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Pfu DNA polymerase (+dNTPs), Economy

Catalog No.: BAM-02-021-EX
Size: 200UNIT
Price: ¥8300
$111
catalog info: Catalog 2012-p210
Storage: -20C
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Purity: >98% by SDS-PAGE
Purpose: For cloning, DNA expression and site-directed mutagenesis
component: [Concentration]
2.5 units/ ul , where one unit is defined as the amount of enzyme that can incorporate 10 nmols of dNTPs into an acid-insoluble material in 30 minutes at 72C when activated salmon sperm DNA was used as template/primer.
[Form] 50mM Tris-H

Other:

 Background 
Pyrococcus furiosus DNA polymerase (Pfu DNA polymerase) gene was expressed in E. coli in large quantities and highly purified. The enzyme has thermostable DNA polymerase activity and 3' -> 5' exonuclease (proofreading) activity. The MW is 90 kDa, same as that of the natural Pfu DNA polymerase.
* Pfu DNA polymerase is thermostabe and has low error rates.
* It is suitable for PCR and primer extension reactions that require high fidelity synthesis.
* Pfu DNA polymerase-generatedPCR fragments are blunt-ended.
[Quality Assurance]
Greater than 95% of protein determined by SDS-PAGE (CBB staining). The absence of endonucleases and exonucleases was confirmed.
[PCR Test]
Good amplification result was obtained in PCR reaction using lambda DNA as a template.
[Storage]
Store at -20C



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Fig.1 SDS-PAGE of Pfu DNA polymerase

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Fig.2 Amplification ofλDNA

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