single-stranded DNA binding protein (SSB) - Cosmo Bio Co.,Ltd.

Antibodies

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single-stranded DNA binding protein (SSB)

Catalog No.: BAM-02-042-5EX
Size: 5*200UG
[5 mg / ml]
Price: ¥34500
$460
Description: Recombinant, E.coli
catalog info: Catalog 2012-p213
Storage: -20C
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Immunogen: Escherichia coli
Purity: >98% by SDS-PAGE
Purpose: Functional single-stranded DNA-binding protein for studying DNA replication and recombination
Enhancement of the specificity and yield of PCR
component: [Form]
5 mg /ml in 20mM Tris-HCl (pH 7.6), 200mM NaCl, 1mM dithiothreitol, 1mM EDTA, 50% glycerol

Other:

 Background 
E. coli single-stranded DNA binding protein (SSB) binds to single-stranded DNA with high specificity (1, 2). It is involved in DNA replication and recombination in vivo. The SSB gene was expressed as the recombinant protein in E.coli highly purified. The molecular mass is 18.9 kDa.
[Quality]
Greater than 95% purity as determined by SDS-PAGE (CBB staining)
The absence of endonucleases and exonucleases was confirmed.
[Molecular Mass]
18.9 kDa
[Data Link]
Swiss-Prot P0AGE0



 IMAGE1



Fig.1 SDS-PAGE of E.coli SSB protein

 IMAGE2



Fig.2 Binding activity to single-stranded DNA 0.02 ug/ul of M13mp18ssDNA was incubated with 0 (lane 0), 0.025 (lane 1), 0.05 (lane 2), and 0.1(lane 3) ug/ul of SSB at 37°C for 30 min. and then 10ul aliquot was subjected to electrophoresis in agarose.

Reference:

•  Krauss,G. et al., Biochemistry 20:5346-5352 (1981)
•  Weiner,J.H. et al., J.Biol.Chem. 250:1972-1980 (1975)
 
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