Taq single-stranded DNA binding protein (SSB) - Cosmo Bio Co.,Ltd.

Antibodies

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Taq single-stranded DNA binding protein (SSB)

Catalog No.: BAM-02-044-EX
Size: 100UG
[0.5 mg / ml]
Price: ¥20000
$267
Description: Recombinant, E.coli
catalog info: Catalog 2012-p213
Storage: -20C
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Purity: >98% by SDS-PAGE
Purpose: Stabilizes single-stranded DNA in DNA replication, repair, and recombination
component: [Form]
50mM Tris-HCl (pH 8.0), 200mM NaCl, 0.1mM dithiothreitol, 0.5mM EDTA, 50% glycerol

Other:

 Background 
Thermus aquaticus derived single-stranded DNA binding protein (SSB) is a thermostable protein which binds to single-stranded DNA with high specificity but does not bind well to double-stranded DNA (1). It plays important roles in DNAreplication and recombination (2). Thermus aquaticus SSB gene was expressed in E.Coli in large quantities and the protein was highly purified.
[Molecular Weight]
30.0 kDa, same as that of the natural protein.
[Activity]
Single-stranded DNA binding activity was confirmed
[Quality Assurance]
Greater than 95% of protein determined by SDS-PAGE (CBB staining)
The absence of endonucleases and exonucleases was confirmed.
[Data Link]
UniProtKB/Swiss-Prot Q9KH06 (SSB_THEAQ)
[Storage]
Store at -20C



 IMAGE1



Fig1.SDS-PAGE of Thermus aquaticus SSB

 IMAGE2



Fig.2 Binding activity to single-stranded DNA 0.02 ug/ul of M13mp18ssDNA was incubated with 0(lane0), 0.025(lane1), 0.05(lane2), and 0.1(lane3) ug/ul of SSB at 37°C for 30 min and then 10 ul aliquot was subjected to electrophoresis in agarose.

Reference:

•  Dabrowski S. et al., Protein Expr Purif. 26: 131-138 (2002)
•  Greipel J. et al., In Saenger,W. and Heinemann,U.(eds) pp.61-86 (1989)
 
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