T4 DNA Ligase - Cosmo Bio Co.,Ltd.

Antibodies

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T4 DNA Ligase

Catalog No.: BAM-02-050-EX
Size: 20000UNIT
[1 mg / ml]
Price: ¥3500
$47
Description: Recombinant, E.coli
catalog info: Catalog 2012-p209
Storage: -20C
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Immunogen: Bacteriophage
Purity: >98% by SDS-PAGE
Purpose: For insertion of DNA fragments into vectors and for Linker (or Adaptor) ligation with DNA fragment
component: 10mM Tris-HCl (pH 7.6), 50mM KCl, 0.1mM EDTA, 1mM dithiothreitol, 50% glycerol

Other:

 Background 
Bacteriophage T4 derived DNA ligase catalyzes the formation of phosphodiester bonds between 3'-OH termini and 5'-P termini in duplex DNA or RNA (1). This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. T4 DNA ligase was expressed in E.coli in large quantities and highly purified. MW is 55.3 kDa.



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Fig.1 SDS-PAGE of T4 DNA ligase protein

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Fig.2 DNA ligation activity Ligation of Hind III fragments of λDNA using 1 unit of T4 DNA ligase Incubation at 16°C for 0, 10, 20, 30, and 60 min.

Reference:

•  Weiss B. et al., J. Biol. Chem. 243: 4543-4555 (1968)
 
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