Lambda Protein Phosphatase - Cosmo Bio Co.,Ltd.

Antibodies

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Lambda Protein Phosphatase

Catalog No.: BAM-02-300-EX
Size: 20000UNIT
[400000 U/ mg]
Price: ¥11000
$147
Description: Recombinant, E.coli
catalog info: Catalog 2012-p226
Storage: -70C
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Purity: >98% by SDS-PAGE
Purpose: λ -PPase can be used to release phosphate groups from phosphorylated serine, threonine, tyrosine and histidine residues in proteins. It should be noted that different proteins are dephosphorylated at different rates. Optimal reaction temperature is 30 degrees. Inclusion of protease inhibitor cocktail and shortest incubation time is desired when assays are done with crude samples.
component: [Form]
400 U/ ul λ -PPase in 50mM HEPES (pH 7.5)
100mM NaCl
2mM dithiothreitol
0.1 mM MnCl2
0.1mM EDTA
50% glycerol
0.01% Brij 35.
[Reagents Supplied with Enzyme]
10 x λ -Ppase

Other:

 Background 
λ Protein Phosphatase (λ -PPase) is a Mn+2-dependent protein phosphatase with activity towards phosphorylated serine, threonine, tyrosine and histidine residues. It is the 221 amino-acid product of ORF221 open readingframe on bacteriophage lambda. λ -PPase was expressed as a recombinant protein in E.coli and highly purified. This product is an intact enzyme of high quality without tag.
[Activity]
400 U/ l , where one unit is defined as the amountof enzyme that hydrolyzes 1nmole of p-nitrophenyl phosphate per minute at 30 degrees. Unit definition assays are performed with 50 mM p-nitrophenyl phosphate in λ -PPase buffer, supplemented with 2 mM MnCl2 in a 50 l reaction.[SpecificActivity]400,000 U / mg
[Quality Assurance] Greater than 95% homogeneous protein determined by SDS-PAGE (CBB staining) that contains no detectable protease activity.



 IMAGE1



Fig.1 SDS-PAGE of λ-Ppase

Reference:

•  Cohen PTW & Cohen P Biochem J. 260: 931-934 (1989) PMID: 2548489
•  Zhuo S et al., J. Biol. Chem. 268:17754-17761 PMID: 8394350 (1993)
 
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