cDNA Library, S.cerevisiae Log Phase - Cosmo Bio Co.,Ltd.

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cDNA Library, S.cerevisiae Log Phase

Catalog No.: BAM-02-701-EX
Size: 500NG
Price: ¥33000
$440
catalog info: Catalog 2012-p211
Storage: -20C
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Immunogen: Saccharomyces cerevisiae
Purity: >98% by SDS-PAGE
Purpose: [Application]
PCR screening of known or unknown gene: Prepare the primers for the known or unknown gene (cDNA) and amplify the gene by PCR from this library followed by cloning to an appropriate vector.
component: [Size]
500 ng (40 ng/ ul , 13ul ) in 10 mM Tris-HCl-1mM EDTA (pH 7.5)
[Quality]
1) Number of independent clones: 3.6 x 106
2) Average insert size : longer than 1 kb

Other:

 Background 
This cDNA li brary (pla smid DNA) is constructed from Saccharomyces cerevisiae, strain S288C-d erived poly(A)+ RNA at the log phase by the Linker -Primer method by Prof. H. Nojima of Osa ka University. This
library is unidirectionallycloned by using the oligo (d T)18 linker primer which contains the restriction en zyme site of NotI, and BamHI (BglII)-SmaI adaptor.
The pLZ3 vector (shown below) used in this lib rary can not replicate in S. cerevisiae but contains pUCori for replication in E. coli.
 Application 
PCR screening of known or unknown gene: Prepare the primers for the known or unknown gene (cDNA) and amplify the gene by PCR from this library followed by cloning to an appropriate vector.
Standard amplifying conditions: 35 cycles of PCR reactions using 10-100 ng of cDNA as a template. (Change the quantity of template and the number of cycles depending on the expression rate of mRNA of the objective gene.)



 IMAGE1



Fig. Structure of pLZ3 and the restriction sites. Ars is the region required for replication in S. pombe, and Ori is a plasmid origin for replication in E. coli

Reference:

•  Kobori M et al., Genes Cells 3: 459-475 (1998)
•  Tanaka S. et al., Genes Cells 1, 905-921 (1996)
•  Tougan T. et al., Nucleic. Acids Res., 36(15):e9 2, (2008)
 
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