cDNA Library, Xenopus ; oocyte - Cosmo Bio Co.,Ltd.

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cDNA Library, Xenopus ; oocyte

Catalog No.: BAM-02-711-EX
Size: 500NG
Price: ¥33000
$440
catalog info: Catalog 2012-p212
Storage: -20C
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Purity: >98% by SDS-PAGE
Purpose: [Application]
PCR screening of known or unknown gene: Prepare the primers for the known or unknown gene (cDNA) and amplify the gene by PCR from this library followed by cloning to an appropriate vector. It is useful for large-scale protein production
component: [Size]
500 ng (40 ng/ ul , 13ul ) in 10 mM Tris-HCl-1mM EDTA (pH 7.5)
[Quality]
1) Number of independent clones: 1.1 x 106
2) Average insert size : longer than 1 kb

Other:

 Background 
This cDNA library (plasmid DNA) is constructed from Xenopus oocyte-derived poly(A)+ RNA by the Linker-Primer method by Professor Hiroshi Nojima of Research Institute for Microbial Diseases, Osaka
University. This library is unidirectionally cloned by using the oligo (dT)18 linker primer which contains the restriction enzyme site of Not I, and BamHI (Bgl II)-Sma I adaptor.
The pBA2 vector used in this library has pUC ori which enables replication in E. coli and Ampr as a selection marker.
 Application 
PCR screening of known or unknown gene: Prepare the primers for the known or unknown gene (cDNA) and amplify the gene by PCR from this library followed by cloning to an appropriate vector. It is useful for large-scale protein productions, and preparation of probes, etc.
Standard amplifying conditions: 35 cycles of PCR reactions using 10-100 ng of cDNA as a template. (Change the quantity of template and the number of cycles depending on the expression level of mRNA of the particular gene.)


Reference:

•  Kobori M et al., Genes Cells 3: 459-475 (1998)
•  Sambrook J. et al., CSHL Press (2001)
 
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