HIV-1 Gag p17 - Cosmo Bio Co.,Ltd.

Antibodies

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HIV-1 Gag p17

Catalog No.: BAM-05-003-EX
Size: 20UG
Price: ¥29000
$387
Application: Western Blot
Enzyme Linked Immunosorbent Assay
Dot Blot
catalog info: Catalog 2012-p166
Storage: -20C
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Immunogen: Human Immunodeficiency Virus
Purity: >98% by SDS-PAGE
Purpose: [Application]
> It can be used as p17 antigen in detection of anti-HIV-1 p17 antibody in Western blotting or ELISA.
> It can be used as standard for the quantitative analysis of HIV-1 p17 antigen.
> It can be used in studies of structure and function of AIDS virus as matrix protein that constitutes HIV-1 core.
component: [Purity]
Over 90% by SDS-PAGE (CBB staining)
[Protein concentration]
0.23 mg / ml as measured by BCA method
[Form]
50% glycerol, 20 mM Tris-HCl (pH7.5), 50 mM NaCl, 10mM mercaptoethanol

Other:

 Background 
HIV-1 Gag p17 is the matrix protein of AIDS virus HIV-1 and is processed by the digestion of its precursor Gag p55 by HIV-1 protease. This protein is indispensable to the reproduction of AIDS virus and constitute the essential element of the of AIDS virus particle construction (1). The product is over-expressed as a recombinant protein in E. coli with a plasmid carrying the Gag p17 coding region of HIV-1 virus, subtype B (2), and highly purified by several steps of chromatography(3). Its molecular weight is 17 kD, same as that of p17 purified from AIDS virus particles (Fig 1).



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Fig. SDS-PAGE of HIV-1 p17

Reference:

•  Freed EO, Virology 251:1-15 (1998)
•  Adachi A. et al., J. Virol. 59, 284 (1986)
•  Saito A. et al., Microbiol. Immunol. 39:473-483 (1995)
 
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