HIV-1 Gag p24 - Cosmo Bio Co.,Ltd.

Antibodies

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HIV-1 Gag p24

Catalog No.: BAM-05-006-EX
Size: 100UG
Price: ¥99000
$1320
Application: Western Blot
Enzyme Linked Immunosorbent Assay
Dot Blot
catalog info: Catalog 2012-p167
Storage: -70C
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Immunogen: Human Immunodeficiency Virus
Purity: >98% by SDS-PAGE
Purpose: [Application]
> It can be used as a standard in titration of p24 antigens as it indicates the amount of HIV-1 virus. This measurement is useful for diagnosis of virus infection and assessing the amount of virus during and after treatment.
> It can be used as antigen for Western blotting or ELISA of anti-HIV-1 p24 antibody.
>It can be used in the studies of structure and function of HIV-1 virus as it constitutes HIV-1 core as a capsid protein.
component: [Purity]
Over 90% Purity by SDS-PAGE (CBB staining)
[Protein concentration]
1 mg / ml measured by BCA method
[Form]
50% glycerol, 20 mM Tris-HCl (pH7.5), 50 mM NaCl, 10 mM mercaptoethanol
[Measurement of the activity]
The ED50 as determined by a cell proliferation assay using MTS assay kit (CellTiter 96, Promega) with human keratinocytes JCRB141 cells was <10 ng/ml.

Other:

 Background 
HIV-1 Gag p24 is a capsid protein that constitutes the core of AIDS virus, HIV-1, and is produced by digestion of its precursor, Gag p55, by HIV-1 protease. This protein is indispensable for reproduction of AIDS virus and constitutesanessential element in the virus particle (1). As this protein is detectable from the early stage of AIDS virus infection, and reflects the amount of virus in the blood, it is used as a marker for observing the patient's condition during and after treatment.
This protein was over-expressed as a recombinant protein in E. coli with a plasmid carrying the Gag p24 coding region of HIV-1 virus, subtype B (2), and highly purified by several steps of chromatography (3). Its molecular weight is 24kD,same as that of p24 purified from HIV-1 virus particles (Fig 1).



 IMAGE1



Fig.1 Polyacrylamide gel electrophoresis of HIV-1 p24 protein

Reference:

•  Freed EO, Virology 251:1-15 (1998)
•  Adachi A. et al., J. Virol. 59, 284 (1986)
•  Saito A. et al., Microbiol. Immunol. 39:473-483 (1995)
 
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