DNA Polymerase κ - Cosmo Bio Co.,Ltd.

Antibodies

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DNA Polymerase κ

Catalog No.: BAM-10-105-EX
Size: 50UG
Price: ¥49000
$654
Description: Recombinant, C-Terminal
Application: Western Blot
Enzyme Linked Immunosorbent Assay
catalog info: Catalog 2012-p162
Storage: -70C
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Immunogen: Human
Purity: >98% by SDS-PAGE
Purpose: [Application]
> Functional studies on translesion DNA synthesis by DNA polymerase kappa
> Antigen for western blotting and ELISA by anti-Pol kappa antibody
component: [Conditions of measurement]
50 mM Tris-HCl (pH 8.0), 2 mM MgCl2, 1 mM DTT, 0.1 mg/ml BSA, 0.1 mM dNTP, 100 nM primer/template (13-mer/30-mer) DNA polymerase 13 - 50 ng /25 ul assay, at 37C, 10 min (Fig. 1)
[Purity]
Over 90% by SDS-PAGE

Other:

 Background 
Mammalian DNA polymerase K, a member of the UmuC/DinB nucleotidyl transferase superfamily, has been implicated in spontaneous mutagenesis (1). Human DNA polymerase K copies undamaged DNA with average single-base subsitution and deletion error rates of 7 x 10-3 and 2 x 10-3, respectively. These error rates are high when compared to those of most other DNA polymerases (2). DNA polymerase K has important role in the mutagenic bypass of certain types of DNA lesions (3).
This product was over-expressed as a recombinant protein in E. coli with a plasmid carrying a C-terminal histidine-tagged human DNA polymerase K (1-560 aa), and highly purified by several steps of chromatography (2). The product is catalytically active and its molecular weight is 65 kD (Fig 1). Activity of this product has been confirmed by a user researcher even if it was diluted 8,000-fold.
[Data Link]
U UniProt KB/Swiss-Prot Q9UBT6 (POLK_HUMAN)
[Storage]
Store at -20C



 IMAGE1



Fig. 1.DNA polymerizing activity of Pol kappa (1-560 aa): 50 mM Tris-HCl (pH 8.0), 2 mM MgCl2, 1 mM DTT, 0.1 mg/ml BSA, 0.1 mM dNTP, 100 nM primer/template (13-mer/30-mer) DNA polymerase κ 13 - 50 ng/25 ul assay, at 37°C, 10 min

 IMAGE2



Fig.2 polyacrylamide gel electrophoresis of DNA polymerase κ protein

Reference:

•  Friedberg EC et al., Proc Natl Acad Sci USA 97: 5681-5683 (2000) PMID: 10811923
•  Ohashi E et al.,
 
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