Fig.1. Methylation of chloroplast DNA visualized by immunochemistry. Samples are Chlamidomonas me-1 cells. Left: DAPI-stained cells. Middle: Cells stained with anti-5MeC antibody and FITC-conjugated 2nd antibody. Right: Merged image. Chloroplast DNA is exclusively methylated in gamete cells
Fig. 2. Detection of DNA methylation in mouse embryonic stem
cells by immunofluorescece staining with the anti-5MeC antibody.
Intense 5-methylcytosine staining at pericentromeric regions was
seen in the mitotic chromosome and interphase nuclei of ESCs.
(For details, see Reference 1.).