Fig.1. Western blot analysis of various mouse
tissues with TRA54 antibody.
For each tissue homogenate, 100 ug protein was applied to each lane. Antibody was used at 1 ug/ml.
Fig. 2. Immunohistochemical staining of mouse testicular cross-section with TRA54 antibody.
Testis from C57BL/6 adult mouse was fixed in Bouinfs solution, followed by paraffin embedding and sectioning. After deparaffinized, sections were treated with normal goat serum, reacted with TRA54 antibody and incubated with biotinylated sheep anti-rat Ig antibody and biotin peroxydase/avidin, followed by incubation with hydrogen peroxide and diaminobenzidine.