Anti ATF6 α - Cosmo Bio Co.,Ltd.

Antibodies

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Anti ATF6 α Antibody

Catalog No.: BAM-73-505-EX
Size: 100UG
Price: ¥42100
$562
antigen/source: ATF6 α
host: Mouse
Purity: IgG fraction
Application: Western Blot
Immunoprecipitation
catalog info: Catalog 2012-p9
Storage: -20C
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Isotype: IgG1
Clone: 37-1
Reacts with: Human
Mouse
Shape: Steri. and Liq.



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Fig.1 Western blot analysis of human cell extracts using this antibody: Conversion of pATF6α(P) to pATF6α(N) in DTT- or tunicamycin-treated cells. 1) untreated 2) DTT: 1mM dithiothreitol (reducing reagent) for 1 h. 3) Tm: 2 µg/ml tunicamycin (inhibitor of N-glycosylation) for 3 h. 4) Tm: 2 µg/ml tunicamycin (inhibitor of N-glycosylation) for 7 h. The asterisk denotes an unglycosylated form of pATF6α(P). ATF6αis constitutively expressed as pATF6α(P) (~90-kDa protein), and converted to pATF6α(N) (>50-kDa protein) in ER-stressed cells.

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Fig.2 Western blot analysis of mouse cell extracts using this antibody: Conversion of pATF6α(P) to pATF6?(N) in DTT- or tunicamycin-treated cells. 1) untreated. 2) DTT: 1mM dithiothreitol for 1 h. 3) Tm: 2 µg/ml tunicamycin for 3 h. 4) Tm: 2 µg/ml tunicamycin for 7 h. The asterisk denotes an unglycosylated form of pATF6α(P). ATF6αis constitutively expressed as pATF6α(P) (~90-kDa protein), and converted to pATF6α(N) (>50-kDa protein) in ER-stressed cells.
 
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