TRAP Staining Kit - Cosmo Bio Co.,Ltd.

Antibodies

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TRAP Staining Kit

Tartrate-resistant acid phosphatase staining kit

Catalog No.: PMC-AK04F-COS
Size: 10PLATE
[for 96-well plate x 10]
Price: ¥33600
$448
catalog info: Catalog 2012-p148
Storage: 4C DNF
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Purpose: Detection of Osteoclast
component: Tartrate-containing Buffer (50 mL ) x 1 bottle
Chromogenic Substrate x 10 vials

Other:

 Background 
The TRAP Staining Kit is used for the staining of Tartrate-Resistant Acid Phosphatase in osteoclasts. Bone mass is controlled by the balance between the activity of osteoblasts bone formation and osteoclasts.
Alkaline phosphatase (ALP staining kit, Cat.No.PMC-AK20-COS) and tartrate-resistant acid phosphate are used as markers for osteoblasts and osteoclasts, respectively.
[Protocol (96-well plate format)]
1. Remove culture medium. Wash each well once with 100 ul of PBS.
2. Add 50 ul of the 10% Formalin Neutral Buffer Solution to each well and fix for 5 minutes at room temperature.
3. Wash each well with 250 ul of deionized water. (x 3 times)
4. Dissolve 1 vial of Chromogenic Substrate with 5 ml of Tartrate-containing Buffer.
5. Add 50 ul of Chromogenic Substrates to each well.
6. Incubate at 37C for 20-60 minutes. Adjust incubation time until stained TRAP is clearly showing the result in figure 1.
7. Wash with deionized water to stop the reaction.
Note: Excess incubation will be cause of over staining.



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TRAP staining of Osteoclast

Reference:

•  Sunao T. et al., JOURNAL OF BONE AND MINERAL RESEARCH 15 1477-1488 (2000)
•  BEN AA. SCHEVEN et al., In Vitro Cell. Dev. Biol. July-August. Animal 34 568-577 (1998)
•  Martha J. Somerman, et al., EXPERIMENTAL CELL RESEARCH 216 335-341 (1995)
•  Ichiro Itonaga et al., Biochemical and Biophysical Research Communications 264 590-595 (1999)
•  Hiroshi Takayanagi et al., Nature 408 600-605 (2000)
•  Morinobu A. et al., Arthritis Rheum. 58 2012-2018 (2008)
•  Hase H. et al., Arthritis Rheum. 58 3356-3365 (2008)
•  Cao H. et al., Bone 46 386-395 (2010)
•  Okada Y. et al., Arch. Oral Biol. 55 502-508 (2010)
•  Okamoto A. et al., Eur. J. Oral Sci. 117 238-247 (2009)
 
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