TRAP Staining Kit - Cosmo Bio Co.,Ltd.



TRAP Staining Kit

Tartrate-resistant acid phosphatase staining kit

Catalog No.: PMC-AK04F-COS
Size: 10PLATE
[for 96-well plate x 10]
Price: ¥33600
catalog info: Catalog 2012-p148
Storage: 4C DNF
Purpose: Detection of Osteoclast
component: Tartrate-containing Buffer (50 mL ) x 1 bottle
Chromogenic Substrate x 10 vials


The TRAP Staining Kit is used for the staining of Tartrate-Resistant Acid Phosphatase in osteoclasts. Bone mass is controlled by the balance between the activity of osteoblasts bone formation and osteoclasts.
Alkaline phosphatase (ALP staining kit, Cat.No.PMC-AK20-COS) and tartrate-resistant acid phosphate are used as markers for osteoblasts and osteoclasts, respectively.
[Protocol (96-well plate format)]
1. Remove culture medium. Wash each well once with 100 ul of PBS.
2. Add 50 ul of the 10% Formalin Neutral Buffer Solution to each well and fix for 5 minutes at room temperature.
3. Wash each well with 250 ul of deionized water. (x 3 times)
4. Dissolve 1 vial of Chromogenic Substrate with 5 ml of Tartrate-containing Buffer.
5. Add 50 ul of Chromogenic Substrates to each well.
6. Incubate at 37C for 20-60 minutes. Adjust incubation time until stained TRAP is clearly showing the result in figure 1.
7. Wash with deionized water to stop the reaction.
Note: Excess incubation will be cause of over staining.



TRAP staining of Osteoclast


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