Anti APP δ C31 - Cosmo Bio Co.,Ltd.

Antibodies

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Anti APP δ C31 Antibody

Alzheimer amyloid precursor protein

Catalog No.: BAM-74-110-EX
Size: 100UL
Price: ¥34500
$460
antigen/source: APP δ C31
host: Rabbit
Description: [Immunogen]C-terminal, APP31
Purity: Serum
Application: Western Blot
Enzyme Linked Immunosorbent Assay
catalog info: Catalog 2012-p8
Storage: -20C
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Immunogen: Human
Reacts with: Human
Mouse
Rat
Shape: Liquid



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Fig.1 Western blot analysis of APPΔC31 (ref.3). Human NT2 neurons (neurally differentiated human NT2 embryonic carcinoma cells) were infected with adenovirus vector expressing β-galactosidase (lane 1), wild-type APP (lane 2) or APPΔC31 (lane3). Cell lysates were prepared 48 h after infection, and proteins were analyzed by Western blotting using this antibody (SAC). Neurons overexpressing wild-type APP contained a 96 kD SAC-immunoreactive fragment which was also detected in APPΔC31-overexpressing neurons.

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Fig.2 Immunocytochemical analysis of APPC31: Caspase-3 activation and generation of the caspase-cleaved fragment APPC31 within neurons induced by serum deprivation (ref.3). Neurally differentiated NT2 cells were cultured for 96 h in the absence of fetal calf serum. Cells were triply labeled for the neuronal marker microtubule-associated protein 2 (MAP2), chromosomal DNA (Hoechst), and activated caspase-3 (ACP3; upper panel) or APPC31 (SAC; lower panel). MAP2-immunopositive neurons with apoptotic nuclei (arrows) are intensively immunostained with ACP3 and SAC.
 
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