Anti MAGE-D1 / Dlxin-1 / NRAGE - Cosmo Bio Co.,Ltd.

Antibodies

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Anti MAGE-D1 / Dlxin-1 / NRAGE Antibody

melanoma-associated antigen D1, Dlxin-1, NRAGE

Catalog No.: BAM-74-112-EX
Size: 100UL
Price: ¥34500
$460
antigen/source: MAGE-D1
host: Rabbit
Description: [Immunogen]a.a. 1-775
Purity: Serum
Application: Western Blot
Immunohistochemistry
catalog info: Catalog 2012-p62
Storage: -20C
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Immunogen: Mouse
Clone: MD1
Reacts with: Human
Mouse
Rat
Shape: Liquid



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Fig.1 Western blot analysis using this antibody. Western blot analysis of necdin and MAGE-D1 in mouse embryonal tissues (ref.2). Tissue lysates (20 ug) from E18.5 embryos were separated by SDS-PAGE and immunoblotted with antibodies against necdin, MAGE-D1 (D1) and tubulin. Endogenous ~43-kDa necdin protein was detected almost exclusively in the brain and skeletal muscle whereas endogenous ~85-kDa MAGE-D1 protein was expressed in a ubiquitous manner.

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Fig.2 Immunohistochemistry using this antibody (ref.2). Fluorescence immunohistochemistry for necdin and MAGE-D1 in mouse embryos. Adjacent sections of the forebrain at E12.5 embryo (upper panels) and the hind limb at E14.5 (lower panels) were stained with antibodies against necdin and MAGE-D1. The arrows point to the preplate (upper panels) and the skeletal muscles (lower panels). The arrowheads indicate the ventricular proliferative zone; V, ventricle; C, bone cavity. Necdin and MAGE-D1 were concentrated in the preplate of the forebrain at E12.5 and skeletal muscle tissues in the hind limb at E14.5. In developing neural tube, MAGE-D1 immunoreactivity was distributed in the ventricular zone as well as the marginal zone.

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Fig.3 Immunocytochemistry using this antibody (ref.2). C2C12 cells were cultured under undifferentiated conditions (UD) or under differentiation conditions for 4 days (4D). Cells were fixed and triply stained for myosin heavy chain (MHC), MAGE-D1 (D1), and nuclear DNA with Hoechst33342 (Hoechst). The arrowheads point to differentiated multinucleated myocytes. MHC and MAGE-D1 were distributed predominantly in the cytosol of multinucleated differentiated C2C12 cells.
 
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