Anti HEV (IgG/IgM), EIA - Cosmo Bio Co.,Ltd.

Antibodies

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Anti HEV (IgG/IgM), EIA

Catalog No.: IIM-1Z23
Size: 96TEST
Price: ¥120000
$1600
antigen/source: IgG, IgM
Description: IgG, IgM
catalog info: Catalog 2012-p112
Storage: 4C
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Immunogen: Human
Reacts with: Human
Purpose: This kit is a detection kit of IgG anti-HEV antibody or IgM anti-HEV antibody in human serum
component: Antibody: X
Plate: X
Coating: X
Control: X
Standard: -
Labeling: X
Substrate: X
Others: X
1. Microplate coated with HEV antigen (8 wells/strip x 12) 1 plate
2. Negative control (blue) 0.5 mL x 1 vial
3. IgG positive control (red) 0.5 mL x 1 vial
4. IgM positive control (yellow) 0.5 mL x 1 vial
5. Sample diluent 50 mL x 1 vial
6. Anti-IgG enzyme labeled monoclonal antibody 5 mL x 1 vial
7. Anti-IgM enzyme labeled monoclonal antibody 5 mL x 1 vial
8. Enzyme substrate 5 mL x 1 vial
9. Reaction stopper 5 mL x 1 vial
10. 20x concentrated washing solution 50 mL x 1 vial
11. Plate seal 3 sheets

Other:

 Supplementary 
Sandwich
 Applicable sample 
Serum
[Other]
IgG anti-HEV antibody is detected in sera of patients infected by hepatitis E virus (HEV) in the past, and IgM anti-HEV antibody is detected during the acute stage of infection with HEV.
The detection system of this kit is based on the enzyme immuno assay (EIA) and is made of 2 steps: the antigen-antibody reaction and the enzyme coloring reaction. The first antigen-antibody reaction takes place between the HEV antigen coatedon themicroplate and the anti-HEV antibody in samples while the second reaction takes place between the IgG/IgM anti-HEV antibody bound to the antigen coated on the microplate and the antibody labeled with enzyme (horseradish peroxidase labeled antibody).When the IgG/IgM anti-HEV antibody is present in samples, the first and the second reactions take place. Then, absorbance by color proportional to the amount of the IgG/IgM anti-HEV antibody in samples developed by enzyme reaction is measured.



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Reference:

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•  Rummenie VT, MatsumotoY, Dogru M, Wang Y, Hu Y, Ward SK, Igarashi A, Wakamatsu T, Ibrahim O, Goto E, Luyten G, Inoue H, Saito I, Shimazaki J, Tsubota K, Cytokine 43(2),p200-208 (2008)
 
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