Calcified nodule Staining kit - Cosmo Bio Co.,Ltd.



Calcified nodule Staining kit

Catalog No.: PMC-AK21-COS
Size: 1SET
[for 24 well plate x 10]
Price: ¥47000
catalog info: Catalog 2012-p153
Storage: RT
Purpose: Calcified Nodule Staining kit
component: Substrate-containing Buffer (x100) (60 mL) x 1 bottle
Chromogenic Substrate x 10 vials
*One kit contains regents for 24-well plates x 10


Alkaline phosphatase, osteopontin, and osteocalcin are expressedduring the differentiation of bone marrow cells into mature osteoblasts and osteoblasts form calcium tubercle. Alizarin Red S, an anthraquinone derivative, has been usedtoidentify Calcified nodule, because Calcium forms an Alizarin Red S-calcium complex, and the products turn (became) red. Calcified Nodule Staining kit contains Substrate-containing Buffer composed mainly of Alizarin Red S and Chromogenic Substrate,andprovides a convenient system for staining calcified nodule with ease.


A: 3T3-E1 cells are cultured using Osteogenesis Culture for Osteogenesis Culture kit (Mouse) (Code No. PMC- OGCMO-COS) (35 mm dish) B: Cells in Osteogenesis Culture Kit (Mouse) (PMC-OGC11-COS) (24well plate)