Osteoclast Culture Kit V-4 - Cosmo Bio Co.,Ltd.

Antibodies

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Osteoclast Culture Kit V-4

Catalog No.: PMC-OSC11-COS
Size: 1SET
Price: ¥152400
$2032
catalog info: Catalog 2012-p267
Storage: -20C & Liquid Nitrogen
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Purpose: To evaluate osteoclast formation and activation.
component: Osteoclast Precursor (Frozen, 2.0 x 106 cells) x 4 vials
Wash Medium (Cat. No. PMC-OSCMW-COS) (100 mL) x 1 bottle
Culture Medium (Cat. No. PMC-OSCMR-COS) (50 mL) x 1 bottle
[Components of Media]
PMC-OSCMW-COS and PMC-OSCMR-COS are complete media formulated for optimal culture of rat osteoclast in vitro. These are sterile, liquid basal media (ƒ¿-MEM) which contain essential and non-essential amino acids, vitamins, other organic compounds, trace minerals, inorganic salts, growth factors, hormones, calf serum, and antibiotics. In addition, PMC-OSCMR-COS, which is used to differentiate preosteoclast to mature osteoclast, contains 50 ng /mL M-CSF and 15 ng /mL RANKL.
[Materials required but not provided]
Variable volume pipettes
Culture plate, 96-well, flat bottom

Other:

 Background 
Bone metabolism is composed of balanced osteogenesis and bone resorption. Research studies have shown that bone marrow cells can be differentiated into osteoclasts using M-CSF (Macrophage Colony Stimulating Factor) and RANKL (Receptor Activator of NF kappa B Ligand).
[Derived from]
ICR Mouse Bone Marrow



 IMAGE1



Osteoclast inducted of differentiation with M-CSF/RANKL

 IMAGE2



Pit on the slice of ivory. (HE staining)

 IMAGE3



Pit on the slice of ivory. (SEM picture)

 IMAGE4



Rat Osteoclast

Reference:

•  Takeshita et al.,(2000), J. Bone Miner. Res. 15, 1477-1488.
•  Scheven et al., (1998), In Vitro Cell Dev Biol Anim 34, 568?577.
•  Martha et al., (1995), Exp. Cell Res. 216, 335-341.
•  Itonaga et al., (1999), Biochem. Biophys. Res. Commun. 242, 703-709.
 
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