Osteoclast Culture Kit V-2 - Cosmo Bio Co.,Ltd.

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Osteoclast Culture Kit V-2

Catalog No.: PMC-OSC13-COS
Size: 1SET
Price: see also
#PMC-OSC14CCOS
Size 1
catalog info: Catalog 2012-p267
Storage: -20C & Liquid Nitrogen
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Purpose: To evaluate osteoclast formation and activation.
component: Osteoclast Precursor (Frozen, 2.0 x 106 cells) x 2 vials
Wash Medium (Cat. No. PMC-OSCMW-COS) (50 mL) x 1 bottle
Culture Medium (Cat. No. PMC-OSCMM-COS) (25 mL) x 1 bottle
PMC-OSCMW-COS and PMC-OSCMM-COS are complete media formulated for optimal culture of Mouse osteoclast in vitro. These are sterile, liquid basal media (-MEM) which contain essential and non-essential amino acids, vitamins, other organic compounds, trace minerals, inorganic salts, growth factors, hormones, calf serum, and antibiotics. In addition, PMC-OSCMM-COS, which is used to differentiate preosteoclast to mature osteoclast, contains 50 ng /mL M-CSF and 25 ng /mL RANKL.
Materials required but not provided
H Variable volume pipettes
H Culture plate, 96-well, flat bottom

Other:

 Background 
Bone metabolism is composed of balanced osteogenesis and bone resorption. Research studies have shown that bone marrow cells can be differentiated into osteoclasts using M-CSF (Macrophage Colony Stimulating Factor) and RANKL (Receptor Activator of NF kappa B Ligand).
[Derived from]
ICR Mouse Bone Marrow



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Reference:

•  Takeshita et al.,(2000), J. Bone Miner. Res. 15, 1477-1488.
•  Scheven et al., (1998), In Vitro Cell Dev Biol Anim 34, 568?577.
•  Martha et al., (1995), Exp. Cell Res. 216, 335-341.
•  Itonaga et al., (1999), Biochem. Biophys. Res. Commun. 242, 703-709.
 
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