Anti p53, phospho Ser46 - Cosmo Bio Co.,Ltd.

Antibodies

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Anti p53, phospho Ser46 Antibody

Catalog No.: BAM-71-115-EX
Size: 50UG
[1 mg / mL]
Price: see also
#BAM-71115EX
Size 100
antigen/source: p53
host: Mouse
Label: Unlabeled
Purity: IgG fraction
Application: Western Blot
IHC paraffin embedding section
Enzyme Linked Immunosorbent Assay
catalog info: Catalog 2012-p73
Storage: -20C
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Immunogen: Human
Isotype: IgG1
Clone: 36
Reacts with: Human



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Fig.1. Identification of Ser46-phosphorylated p53 protein by western blotting. Samples: Crude cell extracts of MOLT-4 untreated (left lanes) and treated with adriamycin for 24 h (right lanes). The left panel is the result with our product and the right panel is the one obtained with the product of our competitor. The lower panel is the whole p53 protein identified by omnipotent anti-p53 antibody.

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Fig.2. Kinetics of phosphorylation of p53 at Ser46 after X-ray irradiation . Samples of U2OS cells (human osteosarcoma) were taken at the indicated times after X-ray irradiation at 10 Gy and analyzed by western blotting with anti-p53 p-S46 antibody (#36) and anti-p53 antibody (non BioAcademia).

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Fig.3. Kinetics of phosphorylation of p53 at Ser46 after UV-irradiation. Samples of MCF7 cells (human beast cancer cell line) were taken at the indicated times after UV-irradiation at 20 J/m2 and analyzed by western blotting with anti-p53 p-S46 antibody (#36) and anti-p53 antibody (non BioAcademia).

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Fig.4. Immunohistochemistry of stomach cancer. Formalin/PFU fixed paraffin-embedded section
 
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