Anti p53, phospho Ser315 - Cosmo Bio Co.,Ltd.

Antibodies

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Anti p53, phospho Ser315 Antibody

Catalog No.: BAM-71-117-EX
Size: 50UG
[1 mg / mL]
Price: see also
#BAM-71117EX
Size 100
antigen/source: p53
host: Mouse
Label: Unlabeled
Purity: IgG fraction
Application: Western Blot
IHC paraffin embedding section
Enzyme Linked Immunosorbent Assay
catalog info: Catalog 2012-p73
Storage: -20C
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Immunogen: Human
Isotype: IgG2b
Clone: 18
Reacts with: Human



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Fig.1 Identification of Ser315-phosphorylated p53 proteinby Western blotting. Sample: Crude cell extracts of MCF7 untreated (lane 1)@and treated with nocodazole at 100 ng/ml for 48 h (lane2). The lower panel is the whole p53 protein identified by@omnipotent anti-p53 antibody (DO-1).

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Fig.2 Immunohistochemistry of stomach cancer. (Formalin/PFA-fixed paraffin-embedded section)

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Fig.3. Immunofluorescence staining of p53 phosho-Ser315 in nuclei of HeLa cells subjected DNA damage. 1. HeLa cells were treated with 100 nM Doxorubicin for 24 hr, fixed with 4% paraformaldehyde overnight, permealized with 0.25% Triton X-100 in PBS for 10 min. 2. Incubate cells with 1.5% BSA in PBS for 30 min to block unspecific binding of the antibodies. Incubate the cells with 1/1,000 diluted anti-p53(p-315) antibody in 1% BSA in PBS at 4°C overnight. 3. Incubate cells with the secondary antibody, goat anti-mouse IgG conjugated with Alex 488, at 1/1,000 dilution in 1% BSA for 1 hr at room temperature. 4. Nucleus (DNA) was stained with DAPI
 
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