Anti c-Myc - Cosmo Bio Co.,Ltd.

Antibodies

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Anti c-Myc Antibody

Catalog No.: BAM-71-161-EX
Size: 50UG
[1 mg / mL]
Price: see also
#BAM-71161EX
Size 100
antigen/source: c-Myc, phospho Ser62
host: Mouse
Label: Unlabeled
Purity: IgG fraction
Application: Western Blot
Enzyme Linked Immunosorbent Assay
catalog info: Catalog 2012-p27
Storage: -20C
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Immunogen: Human
Isotype: IgG2b
Clone: 33A12E10
Reacts with: Human



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Fig.1. Identification of cMyc protein whose Ser62 is phosphorylated by Western blotting. Samples:Crude cell extracts of AGS (gastric adenocarcinoma) cells. Scr;scrambled siRNA was introduced into the cells as a negative control. Neg.Contol; Negative control siRNA from Qiagen was transfected. Myc1; siRNA for cMyc was transfected. (The data was provided by Drs.A. Khanna and J. Westermark of University of Tampere. Finland)

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Fig.2. Immunofluorescence staining of cMyc phosho-Ser62 in nuclei of HeLa cells. 1. HeLa cells were fixed with 4% paraformaldehyde overnight, permealized with 0.25% Triton X-100 in PBS for 10 min. 2. Incubate cells with 1.5% BSA in PBS for 30 min to block non-specific binding of the antibodies. Incubate the cells with 1/2,000 diluted anti-cMyc p62 antibody in 1% BSA in PBS at 4°C overnight. 3. Incubate cells with a secondary antibody, goat anti-mouse IgG conjugated with Alex 488, at 1/1,000 dilution in 1% BSA for 1 hr at room temperature. 4. Nucleus (DNA) was stained with DAPI
 
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