BroadCheck Metallothionein kit - Cosmo Bio Co.,Ltd.

Antibodies

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BroadCheck Metallothionein kit

Catalog No.: FRL-77600-EX
Size: 1KIT
Price: ¥168000
$2240
antigen/source: Metallothionein
Description: Metallothionein
catalog info: Catalog 2012-p112
Storage: 4C
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Reacts with: Human
Animal
Measurement Range: 0.7 - 10000 ng /mL
Purpose: This kit is high sensitivive ELISA Kit by MT antibody for determining human and animal MT. The epitope of this antibody is located at NH2terminals acetylated peptides in MT, therefore can assay native MT.In addition, NH2 terminals of MT is high conserved sequence in many animal species, therefore almost animals MT can measure in this ELISA kit.
component: Antibody: X
Plate: X
Coating: X
Control: -
Standard: X
Labeling: X
Substrate: X
Others: X
1. Antigen coated plate
2. MT Standard
3. Standard diluent
4. Antibody solution
5. Second antibody solution
6. Second antibody diluent
7. Enzyme substrate
8. Stop solution
9. 10X Conc.washing solution
10. Plate seal

Other:

 Supplementary 
Competitive
 Applicable sample 
Blood, organization, urine, cultured cell etc.
[Other]
Metallothionein (MT) which is Cd binding protein have discovered from horse kidney by Margoshe and Vallee in 1957. MT is present in all animalcells and has strong affinity against another heavy metal. The physiological role are concerned in detoxication of organic metal, metabolic regulation of bioessential metal, protection from stress and free radical . However, to obtain high affinityanti MT antibody was very hard because MT is cysteine rich (about30%), low molecular weight protein and by the absence of aromatic amino acid in this structure. Therefore, the high sensitive ELISA system has not established and difficult to investigate the behavior of MT in vivo. We developed anti MT antibody andestablished the high sensitivive ELISA system for determining human and animal MT. The epitope of this antibody is located at NH2 terminals acetylated peptides in MT, therefore can assaynative MT. In addition, NH2 terminals of MT is high conservedsequence in many animal species, therefore almost animals MT can measure in this ELISA kit.
 Features 
The kit is for quantitative detection and the procedure of this assay is simple,easy and hardly influenced by physiological active substancesand body fluid. The epitope of this antibody we used in this kit located at NH2 terminals and acetylated peptides in MT. MT I and II which have commonly NH2 terminals sequence can reactedagainst this antibody. .
[Specificities]
The antibodyinthis kit can recognizethe N terminals, especially acetyl group of N terminal amino acid. Therefore, the cross reactivity against recombinat MT protein expressed in bacteria are under 5%. .
[Assay principal]
This ELISA kit adopts indirectcompetitive reaction usingtherabbit MT polyclonal antibody which recognize the N terminals of MT. The MT are coated in the surface of 96 wells plate, onto which standard of MT or samples to be measured and anti MT antibody are overlaid and incubate. After a washing step, HRP-conjugatedanti rabbit antibody is added and incubate again. HRP conjugated antibody and anti MT antibody - MT complex are formed. After a washing, substrate is added, whichreacts with the HRP conjugate to produce blue colors.More blue color meanslessMT. The optical densities are measured and plotted to calculate the exact concentration of MT.


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