Fig.1 Methylation of chloroplast DNA visualized by immunochemistry. Samples are Chlamidomonas me-1 cells.
Left: DAPI-stained cells
Middle: Cells stained with anti-5MeC antibody and FITC-conjugated 2nd antibody
Right: Merged image
Chloroplast DNA is exclusively methylated in gamete cells.
Fig.2 Detection of DNA methylation in mouse embryonic stem cells by immunofluorescence staining with the anti-5MeC antibody
Intense 5-methylcytosine staining at pericentromeric regions was seen in the mitotic chromosome and interphase nuclei of ESCs (For details, see Reference 1).