HBsAg Subtype EIA - Cosmo Bio Co.,Ltd.



HBsAg Subtype EIA

Catalog No.: IIM-1A63
Size: 24TEST
Price: ¥96000
antigen/source: Subtypes of hepatitis B surface antigen (HBsAg)
Description: Hepatitis B subtypes (d, y, r, w)
catalog info: Catalog 2012-p111
Storage: 4C
Immunogen: Human
Reacts with: Human
Purpose: Determination of subtypes of hepatitis B surface antigen (HBsAg) in serum or plasma
component: Antibody: X
Plate: X
Coating: X
Control: X
Standard: -
Labeling: X
Substrate: X
Others: X
1. Microplate coated with anti-HBs a (8 wells/strip x 12) 1 plate
2. HBsAg negative control 1 mL x 1 vial
3. HBsAg positive control (d) green 0.5 mL x 1 vial
4. HBsAg positive control (y) yellow 0.5 mL x 1 vial
5. HBsAg positive control (r) red 0.5 mL x 1 vial
6.HBsAg positive control (w) white 0.5 mL x 1 vial
7. Enzyme labeled monoclonal antibody (d) green 1.5 mL x 1 vial
8. Enzyme labeled monoclonal antibody (y) yellow 1.5 mL x 1 vial
9. Enzyme labeled monoclonal antibody (r) red 1.5 mL x 1 vial
10. Enzyme labeled monoclonal antibody (w) white 1.5 mL x 1 vial
11. Enzyme substrate 10 mL x 1 vial
12. Reaction stopper 10 mL x 1 vial
13. 20x concentrated washing solution 25 mL x 2 vials
14. Plate seal 5 sheets


[Features and Advantages]
Subtypes of HBsAg can be determined by solid-phase sandwich EIA (Enzyme Immuno Assay)
96-well (8 wells/strip x 12) solid phase microplate coated withanti-HBs a
Total reaction time is 5.5 hours
This kit has been developed as a reagent for research purposes to detect respective subtypic determinants, d, y, r, and w, in hepatitis B surface antigen (HBsAg) positive samples for identification of HBsAg subtypes such as adr, adw, ayr and ayw.
Detection of subtypic determinants is based on the solid-phase sandwich EIA (Enzyme Immuno Assay). 96 wells of the microplate are coated with monoclonal antibody against the common determinant a of HBsAg. HBsAg in samples dispensed to wells is caught on the solid phase and their subtypic determinant, d, y, r, or w, is detected by peroxidase-labeled monoclonal antibody against corresponding determinant




•  Mikhail S, Balayan MD, Int J Infect Dis 2 (2): 113-120 (1997)
•  Mizuo H, Suzuki K, Takikawa Y. et al., J ClinMicrobiol 40: 3209-3218 (2002)
•  Takahashi M, Nishizawa T, Miyajima H. et al., J Gen Virol 84: 851-862 (2003)
•  Tkahashi M, Kusakai S, Mizuho H. et al., J Clin Miclobiol 43: 49-56 (2005)