High Sensitivity CPD ELISA kit - Cosmo Bio Co.,Ltd.

Antibodies

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High Sensitivity CPD ELISA kit

Catalog No.: CSR-NM-MA-K001
Size: 1KIT
[96 TEST]
Price: [DISCONTINUED] Sorry!! This product is not available anymore.
antigen/source: Cyclobutane Pyrimidine Dimers (CPDs)
Storage: 4C
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Reacts with: Human
Animal
component: Antibody: X
Plate: X
Coating: X
Control: -
Standard: X
Labeling: X
Substrate: X
Others: X
1. PROTAMINE SULFATE COATED ELISA PLATE 96 : (8X12) 1 plate
2. Positive CPD Standard using Calf thymus DNA(UVC irradiation : 0 J/m2) : 10 ug / mL , 100 uL /vial ,1 vial (Lyophilized)
3. Assay Diluent (10X) : 10 mL , 1 bottle (Liquid)
4. Wash Buffer (20X) : 15 mL , 2 bottles (Liquid)
5. Blocking Reagent (50X) : 200 uL /vial , 2 vials (Lyophilized)
6. Anti-CPDs (100X) : 150 uL /vial , 1 vial (Lyophilized)
7. Biotinylated 2nd antibody (100X) : 150 uL /vial , 1 vial (Lyophilized)
8. Streptavidin-peroxidase (100X) : 150 uL /vial , 1 vial (Lyophilized)
9. OPD Tablet (2mg) : 2 tablets
OPD Diluent (10X) : 600 uL , 2vials (Liquid)
10. Stop Solution : 12 mL , 1 bottle (Liquid)
11. Cover Film : 3 sheets
Instruction manual : 1

Other:

 Supplementary 
Sandwich
 Applicable sample 
DNA
 Background 
Cyclobutane pyrimidine dimers (CPDs) ELISA kit is optimized for high sensitivity detection of CPDs in DNA purified from cultured cells or from the skin epidermis using an enzyme-linked immunosorbent assay (ELISA). This system can detect CPDs formed in every dipyrimidine sequence (TT, TC, CT and CC) in DNA. Thus, this technology will contribute to understanding the molecular mechanisms of cellular responses to UV light and DNA damage in many research fields including cancer research, photobiology, dermatology, ophthalmology, immunology, and cosmetology.
[Princeple]
To measure DNA damage in DNA, we use ELISA (enzyme-linked immunosorbent assay) using Anti-CPDs (Clone: TDM-2). Genomic DNA is purified from UV-damaged cells and heat denaturedDNA is coated on wells of 96-well plate. The binding of TDM-2 to DNA damage is detected by sequential treatment with biotinylated 2nd antibody and streptavidin-peroxidase. Then, the absorbance of colored products derived from OPD is measured at 492nm.




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Reference:

•  Mori, T. et al., Photochem. Photobiol. 54, 225-232 (1991)
•  Matsunaga, T. et al., Photochem. Photobiol. 54, 403-410 (1991)
•  Potten, C.S. et al., Int. J. Radiat. Biol. 63, 313-324 (1993)
•  Kobayashi, N. et al., J. Invest. Dermatol.101,685-689 (1993)
•  Todo, T. et al., Nature 361, 371-374 (1993)
•  Nakane, H. et al., Nature 377, 165-168 (1995)
•  Komatsu, Y. et al., Nucleic Acids Res. 25, 3889-3894 (1997)
8) Kobayashi, N. et al., J. Invest. Dermatol. 110, 806-810 (1998)
•  Nakagawa, A. et al., J. Invest. Dermatol. 110, 143-148 (1998)
•  Otoshi, E. et al., Cancer Res. 60, 1729-1735 (2000)
•  Katsumi, S. et al., J. Invest. Dermatol. 117, 1156-1161 (2001)
•  Kobayashi, N. et al., Pigment Cell Res. 14, 94-102 (2001)
>Wakasugi M. et al., J. Biol. Chem., 277, 1637-1640 (2002).
•  Imoto, K. et al., J. Invest. Dermatol. 119, 1177-7782 (2002)
•  Nishiwaki Y. et al., J. Invest. Dermatol. 122, 526-532 (2004)
•  Sugasawa K. et al., Cell 121,387-400 (2005).
•  Yasuda G. et al., Mol. Cell. Biol., 27, 6606-6614 (2007).
•  Matsumoto M. et al., J. Cell Sci., 120, 1104-1112 (2007).
•  Yamamoto A. et al., DNA Repair, 6, 649-657 (2007).
 
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