Albumin Glycation Assay Kit, Glyceraldehyde - Cosmo Bio Co.,Ltd.

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Albumin Glycation Assay Kit, Glyceraldehyde

Catalog No.: CSR-AAS-AGE-K01E
Size: 1KIT
[96*2 tests]
Price: ¥45000
$600
Storage: 4C
 
Purpose: For rapid detection of fluorescent AGEs and inhibition assay for glycation of albumin solution by glyceraldehyde.
component: Bovine Serum Albumin(BSA)Solution :10 mL
Glyceraldehyde Solution(500 mM) : 2 mL
Dilution Buffer : 30 mL %Aminoguanidine Solution(20 mM)[Glycation Standard] :0.5 mL
* One kit contains regents for 192 assays (Tube or 96 well Plate)
* Additional aterials required
-96 well black plate(clear bottom、sterile)
Greiner [μCLEAR−PLATE BLACK Cat.No.655090] is recommended.
-Fluorescent microplate reader
(Mode: Fluorescence Bottom Reading, Excitation Wavelength: 370 nm, Emission Wavelength: 440 nm)

Other:

 Background 
The non-enzymatic reaction of reducing carbohydrates with lysine side chains and N-terminal amino groups of macromolecules (proteins, phospholipids and nucleic acids) is called the Maillard reaction or glycation. The products ofthisprocess, termed advanced glycation end products (AGEs), adversely affect the functional properties of proteins. Many AGEs have fluorescent and convalent cross-linking properties. Accumulation of AGEs has been thought to play an important rolein thepathogenesis of diabetic patients as well as the aging process.
Recent studies have suggested that AGEs can arise not only from sugars but also from carbonyl compounds derived from the autoxidation of sugars and other metabolic pathways. Among different AGEs, there is evidence that glyceraldehyde -derived AGEs are associated with such cytotoxcity.
Albumin Glycation Assay Kit provides rapid detection of fluorescent AGEs and inhibition assay for glycation of albumin solution by glyceraldehyde. This kit provides sufficient reagents to perform up to 192 assays.
 Principle 
Albumin Glycation Assay Kit is a complete assay system designed to measure the fluorescent AGEs using the fluorescence microplate readerequipped with a370nmexcitation filter and 440nm emission filter.



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Figure 1. Possible routes of the advanced glycation end-products (AGEs) formation

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Reference:

•  Nishikawa, T. et al., Exp. Cell Res. 171, 164?177. (1987)
•  H. Shoda et al., Endocrinology (1997) 138, 1886?1892.
•  J. Takino et al., J Gastroenterol. 45, 646-655 PMID: 20084527. (2010)
 
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