HBV Pre-S1 Antigen Quantitative ELISA Kit (High Sensitivity) - Cosmo Bio Co.,Ltd.



HBV Pre-S1 Antigen Quantitative ELISA Kit (High Sensitivity)

Catalog No.: BEC-BCL-S1H-01
Size: 1KIT
Price: [DISCONTINUED] Sorry!! This product is not available anymore.
antigen/source: HB Pre-S1
Description: Recombinant, Yeast
catalog info: Catalog 2012-p112
Storage: 4C
Immunogen: Human
Reacts with: Human
Sensitive: 0.1 n Unit / mL
Measurement Range: 0.1- 30 n Unit /mL
Purpose: The kit can be used to determine Pre-S1 antigen in human serum or plasma.
component: Antibody: X
Plate: X
Coating: X
Control: -
Standard: X
Labeling: X
Substrate: X
Others: X
1. Capture IgG (anti-Pre-S1): 120 uL (50 uL/96-well plate)
2. Detection IgG (HRP labeled, anti-Pre-S1): 25 uL (10 uL/96-well plate)
3. Standard antigen (lyophilized form, recombinant antigen): 30 ug/tube
4. Coating Buffer: 25 mL (10 mL/96 well plate)
5. Blocking Agent: 0.2g x 3 (powder, 0.2 g dissolved in 20 mL PBS is enough for 96-well plate)
6. Dilution solution for Detection IgG: 25 mL (10 mL/96-well plate)
7. 20 x PBS: 25 mL
8. 20 x PBS-T: 25 mL
9. Chromogenic Reagent: 25 mL (light shield)i10 mL/96-well platej
10. Stop Solution: 15 mL
11. Microplates (96-wells, split type): two plates
12. User's manual


 Applicable sample 
Serum, Plasma
Three types of hepatitis B virus surface antigen (HBsAg), L-, M-, and S-proteins are known. The L-protein is composed of S, Pre-S2, and Pre-S1 domains, and the M-protein iscomposed of S, Pre-S2 domains, and the S-protein S domain alone. L-protein plays an important role in HBV infection to human hepatic cells, where Pre-S1 region is the key for recognizing human hepatic cells. The "HBs Pre-S1 Quantitative Kit, high sensitivity" enables to explore the human hepatic cell recognizing activity of HBsAg and the HBV infectivity by determining Pre-S1 antigens.
The Pre-S1 region of surface antigen of HBV is known to play an important role in the recognition of human hepatocyte and resulting infection. We now commercialized a new kit that enables to quantitatively detect the Pre-S1 antigen with high sensitivity.
The key components of the kit are two anti-Pre-S1 mouse monoclonal antibodies and Pre-S1antigen. So-called sandwich system is employed, where antibody A captures the antigen on the microplate surface, and the captured antigen is detected by another antibody, antibody B which is labeled with HRP. Finally the amount of HRP attached was determined using chromatic substrate.