Subcutaneous Adipocyte Culture Kit V-1 - Cosmo Bio Co.,Ltd.

Antibodies

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Subcutaneous Adipocyte Culture Kit V-1

Catalog No.: PMC-SAC01-COS
Size: 1SET
Price: ¥135000
$1800
catalog info: Catalog 2012-p266
Storage: -20C & Liquid Nitrogen
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Purpose: Useful for drug development of obesity/diabetes/hypertension/arteriosclerosis, function test of anti-obesity foods, lipid metabolism analyses etc.
component: Subcutaneous White Preadipocytes (Frozen, 3~106 cells) x 1 vial
White Adipocyte Culture Medium (250 mL) x 1 bottle (Cat. No. PMC-SACMR-COS)

Other:

 Background 
White adipocytes, a type of subcutaneous adipocytes, play an important role in energy storage. White Adipocyte Culture Kit, V-1 (Rat) contains preadipocytes isolated from mouse subcutaneous adipose tissues and culture medium that induces differentiation of precursor cells into mature adipocytes. The kit provides a convenient system for studying the mechanism of adipogenesis as well as for analyzing effects of drugs on metabolic syndrome such as obesity, diabetes and hypertension.
[Derived From]
SD Rat Waist Subcutaneous White Adipose Tissue
 Feature and advantages 
Search for obesity, diabetic, high blood pressure, and drug for arteriosclerosis
Development of preventive food of lifestyle-related disease
Functional test of antiobestic functional food
Lipid metabolic experiment
Thermal energy release experiment
Function clarification of Brown Adipocyte
Screening of new 3 agonist
 Protocol 

1. Thaw Adipocyte Culture Medium in a 37C water bath with gentle shaking.
2. Quickly place Common Precursor Cell vial in a 37C water bath until the contents are thawed.
3. Transfer thawed cells into a 15 mL centrifuge tube containing 10 mL of Adipocyte Culture Medium.Mix gently and centrifuge at 4C at 170 x g for 5 minutes.
4. After removing the supernatant, re-suspend cells in 10 mL of Adipocyte Culture Medium and centrifuge at 4C at 170 x g for 5 minutes.
5. After removing the supernatant, re-suspend thecell pellet in 12.5 mL of Adipocyte Culture Medium.
6. Dispense 0.5 mL of cell suspension to each well of 24-well plate.
7. Incubate the plate at 37C under 5% CO2, 100% humidity.
8. After 1 Day culture, add 0.5 ml of Adipocyte Culture Medium gently to each well of 24-well plate.
9. Change the medium every 2 days. Be gentle not to disturb the cell layer.
i. Approximately 3 days of culture, preadipocyte culture becomes confluent.
ii. Approximately 4-5 days of culture, cells turn to mature adipocytes.
iii. Approximately 7 days of culture, cells become hypertrophic.
iv. Approximately 8 days of culture, start detaching from the bottom of the well.



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Reference:

•  Hashimoto, T. _IIIet al., J. Lipid Res. 50 602-610 (2009)
•  Takahashi, K. _IIIet al., PLoS One. 4 e4104 (2009)
•  Oguri, A. _IIIet al., Am. J. Physiol. Cell Physiol. 298 C1414C1423 (2010)
 
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