Taq DNA polymerase Economy (+dNTPs) with Enhancer for High GC template and Robust buffer - Cosmo Bio Co.,Ltd.

Antibodies

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Taq DNA polymerase Economy (+dNTPs) with Enhancer for High GC template and Robust buffer

Catalog No.: BAM-02-003-EX
Size: 200UNIT
Price: ¥5200
$70
Description: Recombinant, E.coli
catalog info: Catalog 2012-p210
Storage: -20C
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Purity: >98% by SDS-PAGE
Purpose: For PCR reactions
component: [Concentration]
5 units/ ul , where one unit is defined as the amount of enzyme that can incorporate 10 nmols of total dNTPs into an acid-insoluble material in 30 minutes at 74C when activated salmon sperm DNA was used as template/primer.
[Form]
20mM Tris-HCl (pH 8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% glycerol, 0.5% Tween20, 0.5% Igepal CA-630

Other:

 Background 
Thermus aquaticus (Taq DNA polymerase) was expressed in E. coli in large quantities and highly purified. The enzyme has thermostable DNA polymerase activity and the MW is 94 kDa. This enzyme kit is especially suitable for PCR reactions with high GC template due to Ehancer for high GC templates and Robust buffer.
 Application 
High-throughput PCR
Colony PCR
Incorporation of dUTP, dITP, and fluorescence-labeled nucleotides
Primer extension
Addition of a single nucleotide (adenosine) at the 3'-blunt ends for cloning into TA vector.



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Fig.1 SDS-PAGE analysis of Taq DNA polymerase

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Fig.2 PCR products obtained by using Robust buffer (agarose gel electrophoresis)

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Fig.3 Effect of the Enhancer on the efficiecy of POR with high GC template (the adenylate cyclase gene from Bordetella pertussis; 67% GC, 6 kb) M Marker 1 without GC Enhancer 2 with GC Enhancer 3 NcoI digestion of the PCR product The adenylate cyclase A gene has a unique NcoI site. The sizes of the digested fragments corresponded to those expected from the physical map.
 
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