Taq DNA polymerase Economy (-dNTPs) with Robust buffer - Cosmo Bio Co.,Ltd.

Antibodies

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Taq DNA polymerase Economy (-dNTPs) with Robust buffer

Catalog No.: BAM-02-012-5EX
Size: 5*200UNIT
Price: ¥16000
$214
Description: Recombinant, E.coli
catalog info: Catalog 2012-p210
Storage: -20C
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Purity: >98% by SDS-PAGE
Purpose: For PCR reactions
component: [Concentration]
5 units/ ul , where one unit is defined as the amount of enzyme that can incorporate 10 nmols of total dNTPs into an acid-insoluble material in 30 minutes at 74C when activated salmon sperm DNA was used as template/primer.
[Form]
20mM Tris-HCl (pH 8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% glycerol, 0.5% Tween20, 0.5% Igepal CA-630

Other:

 Background 
Thermus aquaticus DNA polymerase (Taq DNA polymerase) was expressed in E. coli in large quantities and highly purified. The enzyme has thermostable DNA polymerase activity and the MW is 94 kDa. The enzyme has thermostable DNA polymerase activity and the MW is 94 kDa. This enzyme is suitable for PCR reactions; capable of amplifying DNA with various primers.
 Application 
High-throughput PCR
Colony PCR
Incorporation of dUTP, dITP, and fluorescence-labeled nucleotides
Primer extension
Addition of a single nucleotide (adenosine) at the 3'-blunt ends for cloning into TA vector.



 IMAGE1



Fig.1 SDS-PAGE analysis of Taq DNA polymerase

 IMAGE2



Fig.2 PCR products obtained by using Robust buffer (agarose gel electrophoresis)
 
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