Atelocollagen sponge Mighty 50pcs
||Useful tool for three dimensional culture and research of tissue engineering as 3D scaffold
||Atelocollagen sponge MIGHTY consists primarily of type I atelocollagen derived from bovine dermis and is strong enough to withstand compressive loading of up to 30kPa (single time).
Culturing cells in MIGHTY under cyclic compressive loading (cyclic load of 10 to 20kPa) provides more in vivo-like environment to evaluate cell function. MIGHTY is also applicable as a scaffold for conventional 3D cell culture.
Atelocollagen is a collagen solubilized by protease, but its physical properties are virtually identical to those of natural, unsolubilized collagen. Furthermore, atelocollagen additionally has superior characteristics
- Made from highly purified type I atelocollagen derived from bovine dermis.
- Type I collagen is a major extracellular matrix and provides a scaffold for cultured cells.
- Stands well to cyclic compression loading (repeated 10-20kPa load) andmaintains constant strength.
- Applicable to a scaffold for three dimensional culture
- Suitable for 96-well plate
Atelocollagen is a highly purified type I collagen of calf dermis with protease treatment.Acollagen molecule has an amino acid sequence called a telopeptide at both N and C termini, which confers most of the collagen's antigenicity. Atelocollagen obtained by protease treatment is low in immunogenicity because it is free from telopeptides.
Atelocollagen is generally obtainable with a high degree of purity. This feature is due to the protease treatment, which when used to extract atelocollagen breaks down other protein contaminations.
Atelocollagen is biodegradable. Therefore, atelocollagen is used in a variety of fields such as medicine, medical devices and cosmetics as a raw material, and research in cell culture.
Atelocollagen can be engineered into manydifferent physicalshapes such as films, sponge-like structure, string-like configurations, powders and gels. It is possible to leverage the special properties of atelocollagen to produce the most appropriate configuration for any application. For example,atelocollagen is normally insoluble in water with neutral pH, but this characteristic can be altered to make it soluble. It is also possible to use atelocollagen equally as a coagulant or as an anticoagulant, and it is also possible to controltherate at whichit is absorbed by the body.
KOKEN ensures the safety of materials through the precise management of each and every bovine used as a source of material for atelocollagen production;
1.The bovine dermis we use comesfrom Australiawhich has a national livestock traceability system in place, and we only use calves aged six months or younger.
2.We do not use animal feed derived from bovine and sheep but only BSE-free, safe feed.
3.We only use the dermal layer ofthe skin,which is classified as belonging to the ''no detectable infectivity'' category (WHO Guidelines on Tissue Infectivity Distribution in Transmissible Spongiform Encephalopathies). We take great care to prevent this layer from coming into contactwith hazardoussites including the brain and the spine during collection.
4.We are able to track bovine used for KOKEN's collagen to their birthplace, and we periodically conduct field research
• Muroi Y et al., Effects of compressive loading on human synovium-derived cells. (2007) J Dent Res. 86(8):786-791.