Prolyl 4-Hydroxylated α -Fibrinogen ELISA Kit - Cosmo Bio Co.,Ltd.

Antibodies

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Prolyl 4-Hydroxylated α -Fibrinogen ELISA Kit

Fib2; MGC119422

Catalog No.: KAL-KG612
Size: 1KIT
Price: ¥100000
$1334
antigen/source: α -Fibrinogen
Storage: 4C
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Immunogen: Human
Reacts with: Human
Gene ID:64435
Gene Symbol: FGA
Sensitive: 0.43 ng/mL
Measurement Range: 2.73-175 ng/mL
Purpose: This assay kit is a quantitative sandwich-ELISA-Kit based on two mouse monoclonal antibodies against to different epitope of HP-FGA with hydroxylation on its proline 565 residue.
component: Antibody: X
Plate: X
Coating: X
Control: -
Standard: X
Labeling: X
Substrate: X
Others: X
1. Precoated plate (Anti-Human FGA Mouse IgG MoAb Affinity Purify) : 96 Well x 1
2. Labeled antibody Conc. : (30X) HRP conjugated Anti- Human HP-FGA Mouse IgG Fab' Affinity Purify : 0.4 mL x 1
3. Standard (Human HP-FGA) : 0.5 mL x 2
4. EIA buffer (1% BSA, 0.05% Tween20 in PBS) : 30 mL x 1
5. Solution for Labeled antibody (1% BSA, 0.05% Tween20 in PBS) : 12 mL x 1
6. Chromogen (TMB solution) : 15 mL x 1
7. Stop solution (1N H2SO4) : 12 mL x 1
8. Wash buffer Conc ((40X) 0.05% Tween20 in phosphate buffer) : 50 mL x 1

Other:

 Supplementary 
Competitive
 Applicable sample 
Plasma(EDTA), Culture medium
[Other]
Fibrinogen is one of major component of plasma, and is a hexamer composed of pairs of three polypeptides (-, - and -chain). Prolyl hydroxylation isknown to be essential for folding, secretion, and stability of collagen triple helix. In the powerful study using 2-Dimensional Image Converted Analysis of Liquid chromatography and mass spectrometry (2DICAL), it is found that proline 565 and 530 residues in -fibrinogen is hydroxylated by the Prolyl hydroxylase P4HA1. In addition, the plasma level of prolyl 4-hydroxylated -fibrinogen (HP-FGA) was increased in individuals who suffer pancreatic cancer. The significant difference between the said group (n=43) and healthy subjects (n=43) was observed in the 2-DICAL analysis.



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Reference:

•  Ono M, Matsubara J, Honda K, Sakuma T, Hashiguchi T, Nose H, Nakamori S, Okusaka T, Kosuge T, Sata N, Nagai H, Ioka T, Tanaka S, Tsuchida A, Aoki T, Shimahara M, Yasunami Y, Itoi T, Moriyasu F, Negishi A, Kuwabara H, Shoji A, Hirohashi S, Yamada T. J Biol Chem. 2009 Oct 16;284(42):29041-9.
 
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