Anti D- β -Aspartic acid - Cosmo Bio Co.,Ltd.

Antibodies

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Anti D- β -Aspartic acid Antibody

Catalog No.: CAC-KUF-P001
Size: 100UL
Price: [DISCONTINUED] Sorry!! This product is not available anymore.
antigen/source: D-beta-Aspartic acid
host: Rabbit
Description: [Antigen]Gly-Leu-D-beta-Asp-Ala-Thr-Gly-Leu-D-beta-Asp-Ala-Thr-Gly-Leu-D-beta-Asp-Ala-Thr
Label: Unlabeled
Purity: Affinity Purified
Application: Western Blot
IHC paraffin embedding section
Enzyme Linked Immunosorbent Assay
Storage: -20C
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Shape: Liquid

Other:

 Background 
Biologically uncommon D-aspartyl (Asp) residues have been reported in proteins of the tooth, eye lens, aorta, and
brain in elderly humans. Aspartic acid is the most easily racemizable amino acid and D-Asp may be formed by
racemization in metabolically inactive tissues during the chronological aging process. We prepared a highly specific
polyclonal antibody against peptide Gly-Leu-D--Asp-Ala-Thr-Gly-Leu-D-b-Asp-Ala-Thr-Gly-Leu-D-
-Asp-Ala-Thr (anti-peptide 3R antibody) which corresponds to three repeats of positions 149-153 of human
A-crystallin. This antibody can distinguish the configuration of the Asp-residue, that is, it reacts very strongly with the
D--Asp-containing peptide but not react with the L--Asp-, L--Asp- and D--Asp-containing peptides.
 Application 
Recommended use
WB, IHC (Paraffin), ELISA
Recommended dilutions
Western blotting, 1/300
Immunohistochemistry, It depends on tissue sites.
ELISA, 1/300
Optimaldilutions/concentrations should be determined by the end user.



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Immunoreactivity of the antibody for D-b-Asp-containing peptide (peptide 3R) in the skin. Skin specimens, obtained from the sun-exposed area (face) of 9-year-old (A), 59-year-old (B), and 86-yearold (C), or sun-protected area (buttock) of 85-year-old (D), were embedded in paraffin and then immunoreacted with the antibody for peptide 3R at 1:100 dilution for 24 h. The sections were incubated with rabbit anti-Ig antibody at 1:50 dilution for 2h. Antigen-antibody complex was visualized by avidin?biotin complex (60 in A, B and D, 100 in C).
 
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